José Julio Ortega-Calvo

jose julio

Dr. J. Julio Ortega-Calvo

Investigador científico
Departamento Agroquímica, Microbiología Ambiental y Conservación de Suelos
Instituto de Recursos Naturales y Agrobiologia de Sevilla
Avenida Reina Mercedes 10
41080 – Sevilla (Spain)
tel: +34 95 462 47 11 Ext. 208171

e-mail: jjortega<at>

Environmental Microbiology and Biodegradation: Bacterial mechanisms to increase the bioavailability of organic pollutants in soil. Roles of biosurfactants, adhesion and chemotaxis.

Processes of sequestration of organic pollutants in soil: Effects of partitioning from NAPLs and sorption on biodegradation.

Optimisation of bioremediation: Application of surfactants and phytoremediation.

“Bioremediation and revegetation to restore the public use of contaminated land”. European Union, 2016-2019

“Evaluación de estrategias químicas sostenibles para la mejora de la bioaccesibilidad en la biorremediación de suelos contaminados por PAHs”. Andalusian Government, 2014-2018

“Sinergias funcionales entre microorganismos y plantas en la recuperación sostenible de suelos contaminados por HAPs”. Spanish Ministry of Economy and Competitiveness, 2014-2016.

Explotacion de las funciones microbianas de la rizosfera promotoras de la bioaccesibilidad y biodegradación de HAPs para la recuperación eficiente de suelos contaminados“. Spanish Ministry of Science and Innovation, 2011-2013.

Biorremediación de suelos: prospección de la diversidad microbiana y vegetal para la mejora de la bioaccesibilidad y mineralización de hidrocarburos aromáticos policíclicos“. Spanish Ministry of Education, 2007-2010.

“Biodisponibilidad de hidrocarburos aromáticos policíclicos y sus metabolitos presentes en suelos contaminados sometidos a biorrecuperación”. Spanish Ministry of Environment, 2004-2006.

“Biodisponibilidad y metabolismo microbianos de hidrocarburos aromáticos policíclicos presentes en vertidos marinos de petróleo. Implicaciones para su atenuación natural y biorremediación. Spanish Ministry of Education, 2005-2008.

Evaluation of availability to biota for organic compounds ubiquitous in soils and sediments“.European Union.2001-2004.

Use of bioavailability-promoting micro-organisms to decontaminate PAH-polluted soils:  preparation towards large-scale field exploitation“. European Union & Spanish CICYT. 2000-2004.

Development of biotechnology for soil detoxication from polycyclic aromatic hydrocarbons (PAHs) on the basis of application of chemotactically active microorganisms of plant rhizosphere“. European Union & International Science and Technology Centre (ISTC). 1999-2001.

Evaluation of bacterial strategies to promote bioavailability of hydrophobic pollutants for efficient bioremediation of contaminated soils“. European Union & Spanish CICYT. 1997-2000.

Biotechnology of soil: monitoring, conservation and remediation“. COST Action 831. European Union.1998-2003.

SETAC Europe 20th Special Science Symposium “Bioavailability of organic chemicals: linking science to risk assessment and regulation”. Brussels, 14h -15th October 2014 (

SETAC Europe 20th Annual Meeting “Science and Technology for Environmental Protection”. Seville, 23rd – 27th May 2010 ( ).

4th International Meeting Bioavailability of Pollutants and Soil Remediation. Seville, 10th-13th September, 2006. Main Sponsors: Fundación BBVA and CRC-CARE Australia.

Researcher ID

Ortega-Calvo, J. J., Jimenez-Sanchez, C., Pratarolo, P., Pullin, H., Scott, T.B., Thompson, I.P. (2016) Tactic response of bacteria to zero-valent iron nanoparticles. Environmental Pollution 213:438-445.

Ortega-Calvo, J.J., Harmsen, J., Parsons, J. P., Semple, K., Aitken, M. D., Ajao, C, Eadsforth, C., Malyka Galay-Burgos, M., Naidu, R., Oliver, R., Peijnenburg, W. J. G. M., Römbke, J., Streck, G., Versonnen, B. (2015) From bioavailability science to regulation of organic chemicals. Environmental Science and Technology 49: 10255−10264.

Jimenez-Sanchez, C., Wick, L.Y., Cantos, M., Ortega-Calvo, J.J. (2015) Impact of dissolved organic matter on bacterial tactic motility, attachment, and transport. Environmental Science and Technology 49: 4498−4505.

Congiu, E., Parsons, J.R., Ortega-Calvo, J.J. (2015) Dual partitioning and attachment effects of rhamnolipid on pyrene biodegradation under bioavailability restrictions. Environmental Pollution 205:378-384.

Sungthong, R., van West, P., Cantos, M., Ortega-Calvo, J.J. (2015) Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments: A set of behaviors that are relevant for bioremediation. Science of the Total Environment 511: 767–776.

Congiu, E., Ortega-Calvo, J.J. (2014) Role of desorption kinetics in the rhamnolipid-enhanced biodegradation of polycyclic aromatic hydrocarbons. Environmental Science and Technology 48:10869–10877.

Tejeda-Agredano, M. C., Mayer, P., Ortega-Calvo, J. J. (2014) The effect of humic acids on biodegradation of polycyclic aromatic hydrocarbons depends on the exposure regime. Environmental Pollution 184:435-442.

Martín, V.I., de la Haba, R.R., Ventosa, A., Congiu, E., Ortega-Calvo, J.J., Moyá, M.L. (2014) Colloidal and biological properties of cationic single-chain and dimeric surfactants. Colloids and Surfaces B: Biointerfaces 114: 247– 254.

Ortega-Calvo, J. J., Tejeda-Agredano, M. C., Jimenez-Sanchez, C., Congiu, E., Sungthong, R., Niqui-Arroyo, J. L., Cantos, M. (2013) Is it possible to increase bioavailability but not environmental risk of PAHs in bioremediation? Journal of Hazardous Materials 261: 733– 745.

Krell, T., Lacal, J., Reyes-Darias, J.A., Jimenez-Sanchez, C., Sungthong, R., Ortega-Calvo, J.J. (2013) Bioavailability of pollutants and chemotaxis. Current Opinion in Biotechnology 24:451–456.

 Tejeda-Agredano, M.C., Gallego, S., Vila, J., Grifoll, M., Ortega-Calvo, J.J., Cantos, M. (2013). Influence of the sunflower rhizosphere on the biodegradation of PAHs in soil. Soil Biology and Biochemistry 57:830–840.

 Jimenez-Sanchez, C., Wick, L.Y., Ortega-Calvo, J.J. (2012). Chemical effectors cause different motile behavior and deposition of bacteria in porous media. Environmental Science and Technology 46:6790-6797.

Tejeda-Agredano, M. C., Gallego, S., Niqui-Arroyo, J. L., Vila, J., Grifoll, M., Ortega-Calvo, J. J. (2011) Effect of interface fertilization on biodegradation of polycyclic aromatic hydrocarbons present in nonaqueous-phase liquids. Environmental Science and Technology 45: 1074-1081.

Bueno-Montes, M., Springael, D., Ortega-Calvo, J. J. (2011) Effect of a non-ionic surfactant on biodegradation of slowly desorbing PAHs in contaminated soils. Environmental Science and Technology 45: 3019-3026.

Posada-Baquero, R., Ortega-Calvo, J. J. (2011) Recalcitrance of polycyclic aromatic hydrocarbons in soil contributes to background pollution. Environmental Pollution 159:3692-3699.

Ortega-Calvo, J. J., Molina, R., Jimenez-Sanchez, C., Dobson, P.J., Thompson, I.P. (2011) Bacterial tactic response to silver nanoparticles. Environmental Microbiology Reports 3:526-534.

Lacal, J., Muñoz-Martínez, F., Reyes-Darías, J.A., Duque, E., Matilla, M., Segura, A., Ortega-Calvo, J.J., Jimenez-Sanchez, C., Krell, T. y Ramos, J.L. (2011) Bacterial chemotaxis towards aromatic hydrocarbons in Pseudomonas. Environmental Microbiology 13: 1733-1744.

 Niqui-Arroyo, J.L., Ortega-Calvo, J.J. (2010) Effect of electrokinetics on the bioaccessibility of polycyclic aromatic hydrocarbons in polluted soils. Journal of Environmental Quality 39: 1993–1998.

Ortega-Calvo, J. J., Gschwend, P.M. (2010). Influence of low oxygen tensions and sorption to sediment black carbon on biodegradation of pyrene. Applied and Environmental Microbiology 76:4430-4437.

Lopez, Z.,Vila, J., Ortega-Calvo, J.J., Grifoll, M. (2008). Simultaneous biodegradation of creosote-polycyclic aromatic hydrocarbons by a pyrene-degrading Mycobacterium. Applied Microbiology and Biotechnology 78:165-172.

Velasco-Casal, P., Wick, L.Y., Ortega-Calvo, J.J. (2008). Chemoeffectors decrease the deposition of chemotactic bacteria during transport in porous media. Environmental Science and Technology 42:1131-1137.

Posada-Baquero R, Niqui-Arroyo J-L, Bueno-Montes M, Gutiérrez-Dabán A, Ortega-Calvo J-J (2008). Dual 14C/residue analysis method to assess the microbial accessibility of native phenanthrene in environmental samples. Environmental Geochemistry and Health 30:159-163.

Hafka, J., Parsons, J.R., Govers, H.A.J., Ortega-Calvo, J.J. (2008). Enhanced kinetics of solid-phase microextraction and biodegradation of polycyclic aromatic hydrocarbons in the presence of dissolved organic matter. Environmental Toxicology and Chemistry 27:1526-1532.

Niqui-Arroyo, J.L., Ortega-Calvo (2007) Integrating biodegradation and electroosmosis for the enhanced removal of polycyclic aromatic hydrocarbons from creosote-polluted soils. Journal of Environmental Quality 36:1444-1451.

Uyttebroek, M., Spoden, A., Ortega-Calvo, J.J., Wouters, K., Wattiau, P., Bastiaens, L., Springael, D. (2007). Differential responses of eubacterial, Mycobacterium, and Sphingomonas communities in polycyclic aromatic hydrocarbon (PAH)-contaminated soil to artificially induced changes in PAH profile. Journal of Environmental Quality 36:1403-1411.

Niqui-Arroyo, J.L., Bueno-Montes, M., Posada-Baquero, R. and Ortega-Calvo, J.J. (2006) Electrokinetic enhancement of phenanthrene biodegradation in creosote-polluted clay soil. Environmental Pollution 142:326-332.

Uyttebroek, M., Breugelmans, P., Janssen, M., Wattiau, P. Joffe, B., Karlson, U., Ortega-Calvo, J.J., Bastiaens, L., Ryngaert, A., and Springael, D. (2006) Distribution of the Mycobacterium community and polycyclic aromatic hydrocarbons (PAHs) among different size fractions of a long-term PAH-contaminated soil. Environmental Microbiology 8: 836-847.

Hermosín, M.C., Celis, R., Facenda, G., Carrizosa, M.J., Ortega-Calvo J.J., and Cornejo, J. (2006) Bioavailability of the herbicide 2,4-D formulated with organoclays. Soil Biology and Biochemistry 38:2117-2124.

Uyttebroek, M., Ortega-Calvo, J.J., Breugelmans, P., Springael, D. (2006). Comparison of mineralization of soil-sorbed phenanthrene by polycyclic aromatic hydrocarbon (PAH)-degrading Mycobacterium spp. and Sphingomonas spp. Applied Microbiology and Biotechnology 72:829-836.

Gomez-Lahoz, C. and Ortega-Calvo, J.J. (2005) Effect of slow desorption on the kinetics of biodegradation of polycyclic aromatic hydrocarbons. Environmental Science and Technology 39:8776-8783.

Ortega-Calvo, J. J., A. I. Marchenko, A. I. Borobyov, and R. V. Borovick. (2003) Chemotaxis in polycyclic aromatic hydrocarbon-degrading bacteria isolated from coal tar- and oil-polluted rhizospheres. FEMS Microbiology Ecology 44:373-381.

Resina-Pelfort, O., García-Junco, M., Ortega-Calvo, J.J., Comas-Riu, J. and Vives-Rego, J. (2003). Flow cytometry discrimination between bacteria and clay humic acid particles during growth-linked biodegradation of phenanthrene by Pseudomonas aeruginosa 19SJ. FEMS Microbiology Ecology 43:55 – 61.

García-Junco, M., Gómez-Lahoz, C., Niqui-Arroyo, J.L., and Ortega-Calvo, J.J. (2003). Biosurfactant– and biodegradation-enhanced partitioning of polycyclic aromatic hydrocarbons from Nonaqueous-phase liquids. Environmental Science and Technology 37:2988-2996.

García-Junco, M., De Olmedo, E. and Ortega-Calvo, J.J. (2001) Bioavailability of solid and non-aqueous phase liquid (NAPL)-dissolved phenanthrene to the biosurfactant-producing bacterium Pseudomonas aeruginosa 19SJ. Environmental Microbiology 3:561-569.

Lahlou, M., Harms, H., Springael, D. and Ortega-Calvo, J.J. (2000) Influence of soil components on the transport of polycyclic hydrocarbon-degrading bacteria through saturated porous media. Environmental Science and Technology 34:3649-3656.

Lahlou, M. and Ortega-Calvo (1999) Bioavailability of labile and desorption-resistant phenanthrene sorbed to montmorillonite clay containing humic fractions. Environmental Toxicology and Chemistry 18:2729-2735.

Ortega-Calvo, J.J., Fesch, C., and Harms, H. (1999) Biodegradation of Sorbed 2.4-dinitrotoluene in a clay-rich, aggregated porous medium. Environmental Science and Technology 33:3737-3742.

Ortega-Calvo, J.J., and C. Saiz Jimenez (1998) Effect of humic fractions and clay on biodegradation of phenanthrene by a Pseudomonas fluorescens strain isolated from soil. Applied and Environmental Microbiology 64:3123-3126.

Ortega-Calvo, J.J., Lahlou, M. and Saiz-Jimenez, C. (1997) Effect of organic matter and clays on the biodegradation of phenanthrene in soils. International Biodeterioration and Biodegradation 40:101-106.

Ortega-Calvo, J.J. and C. Saiz-Jimenez (1997) Microbial degradation of phenanthrene in two European Cathedrals. FEMS Microbiology Ecology 22:95-101.

Ortega-Calvo, J.J., Birman, I. and Alexander, M. (1995) Effect of varying the rate of partitioning of phenanthrene in nonaqueous-phase liquids on biodegradation in soil slurries. Environmental Science and Technology 29:2222-2225.

Ortega-Calvo, J.J. and Alexander, M. (1994) Role of bacterial attachment and spontaneous partitioning in the biodegradation of naphthalene initially present in non-aqueous phase liquids. Applied and Environmental Microbiology 60:2643-2646.

Ortega-Calvo, J.J. and Stal, L.J. (1994) Sulfate-limited growth in the nitrogen-fixing unicellular cyanobacterium Gloeothece (Nageli) sp. PCC 6909. The New Phytologist 128:273-281.

Ortega-Calvo, J.J., Sanchez-Castillo, P.M., Hernandez-Marine, M. and Saiz-Jimenez, C. (1993). Isolation and characterization of epilithic chlorophytes and cyanobacteria from two Spanish cathedrals (Salamanca and Toledo). Nova Hedwigia 57:239-253.

Ortega-Calvo, J.J. and Stal, L. (1991). Diazotrophic growth of the unicellular cyanobacterium Gloeothece sp. PCC 6909 in continuous culture. Journal of General Microbiology 137:1789-1797.