Plant Ion and Water Regulation (RIH)

Some members of the RIH’s group also belong to the Plant Molecular Ecophysiology Lab (LEM):
https://www.irnas.csic.es/laboratorio-interdepartamental-de-ecofisiologia-molecular-de-plantas-lem/

DEFINITION

The Plant Ion and Water Regulation group (RIH), led by Dr. José M. Colmenero-Flores, develops two main research lines:

1. Chloride (Cl) nutrition and its functions in higher plants.

Cl has traditionally been considered harmful to crops due to its toxicity under salinity conditions and for impairing nitrate (NO3) nutrition. However, our results have allowed us to classify the Cl as a beneficial macronutrient, due to its role in plant development and the improvement of the efficiency of the use of water, nitrogen and CO2, three basic pillars of plant nutrition.

Therefore, the Cl homeostatic control is meticulously regulated by plants through environmental and developmental factors, in coordination with the NO3 transport and accumulation. We are also working in the identification and characterization of molecular mechanisms that regulate these processes, including anion transporters as CCC, SLAC/SLAH, NPF and NRT2 families, which are involved in Cl homeostasis.

 

Our goal is to transfer this knowledge to agriculture through biotechnological and cultivation approaches to improve resistance to drought and salinity, as well as to reduce the use of NO3 and its excessive accumulation in food.

The European project ChlorPlant (H2020-MSCA-IF-CAR) recently awarded to Dr. Miguel A. Rosales will allow us to delve into how Cl modulates the effects of water management on plant development, photosynthesis, turgor maintenance, fruit production and quality, and resistance to drought in tomato plants. This 3-year project will integrate the leading models of photosynthesis and stomatal conductance into a mechanistic model at the complete plant level, which will include cutting-edge phenotyping techniques. ChlorPlant will address 2 major European challenges with expected agricultural benefits: helping to increase water use efficiency in arid and semi-arid areas affected by climate change, and reducing excessive use and nitrate pollution in agriculture, both in continental waters as for its toxic accumulation in fresh consumption crops (vegetables).

More information: https://cordis.europa.eu/project/id/895613

 

2. Genotypic and phenotypic variability of wild olive genotypes (SILVOLIVE Collection).

The SILVOLIVE Collection of wild olive genotypes has been developed and characterized with the to improve crop resistance and productivity, as well as their interest in mitigating the effects of climate change.

 

 

In both research lines, we collaborate closely and transfer results to companies in the agri-food and nursery sectors.

 

GROUP MEMBERS:

 

Dr. José M. Colmenero Flores

Group Leader – Researcher CSIC

Dr. Miguel A. Rosales Villegas

Doctor – MSCA

Dra. Mª Rosario Álvarez Morales

Profesor Collaborator US

mrosario@us.es

Francisco J. Durán Gutiérrez

Technician R+D+i

Juan D. Franco Navarro

PhD student

Pablo Díaz Rueda

PhD student

Procopio Peinado Torrubia

PhD student

Marta Lucas Gutiérrez

PhD student

mlucas@irnas.csic.es

Francisco J. Moreno Racero

Master student

fjmracero@gmail.com

Adrián Gómez Garzón

Master student

Blanca Beas Santos

Master student

Mª Ángeles Vargas Pérez

Master student

Álvaro F. García Rodríguez

Master student

alvgarciarodriguez@gmail.com

 

 

 

MEMBERS OF THE UNIT OF PLANT CULTURE BIOTECHNOLOGY

 

Miriam Pérez Sayago

Technician Manager

Mª del Pilar Alcántara Romano

Technician PTA

Dr. Isidro Álvarez Escribano

Contract Garantía Juvenil

isidroae9@hotmail.com

Carlos M. Rivero Núñez

Colaborator R+D+i and vulgarization

carlosrivero1986@gmail.com

2016-2020

  • Díaz-Rueda P, Franco-Navarro JD, Messora R, Espartero J, Rivero-Núñez CM, Aleza P, Capote N, Cantos M, García-Fernández JL, de Cires A, Belaj A, León L, Besnard G, Colmenero-Flores JM (2020) SILVOLIVE, a Germplasm Collection of Wild Subspecies With High Genetic Variability as a Source of Rootstocks and Resistance Genes for Olive Breeding. Frontiers in Plant Science 11:629.
  • Rosales MA* & Franco-Navarro JD*, Peinado-Torrubia P, Díaz-Rueda P, Alvarez R, Colmenero-Flores JM (2020) Chloride improves nitrate utilization and NUE in plants. Frontiers in Plant Science 11:442.
  • Gallego-Tévar B, Peinado-Torrubia P, Álvarez R, Gandullo J, Grewell BJ, Figueroa E, Castillo JM (2020) Changes to the functional traits of phosphoenolpyruvate carboxylase following hybridization in C-4 halophytes. Physiologia Plantarum 169:83–98.
  • León L, Díaz-Rueda P, Belaj A, De la Rosa R, Carrascosa C, Colmenero-Flores JM (2020) Evaluation of early vigor traits in wild olive germplasm. Scientia Horticulturae 264:109157.
  • Nieves-Cordones M, García-Sánchez F, Pérez-Pérez J, Colmenero-Flores JM, Rubio F, Rosales MA (2019) Coping with water shortage: An update on the role of K+, Cl, and water membrane transport mechanisms on drought resistance. Frontiers in Plant Science 10:1619.
  • Colmenero-Flores JM, Franco-Navarro JD, Cubero-Font P, Peinado-Torrubia P, Rosales MA (2019) Chloride as a beneficial macronutrient in higher plants: New roles and regulation. International Journal of Molecular Sciences 20:4686.
  • Hernandez-Santana V, Diaz-Rueda P, Diaz-Espejo A, Raya-Sereno MD, Gutiérrez-Gordillo S, Montero A, Perez-Martin A, Colmenero-Flores JM, Rodriguez-Dominguez CM (2019) Hydraulic traits emerge as relevant determinants of growth patterns in wild olive genotypes under water stress. Frontiers in Plant Science 10:291.
  • Franco-Navarro JD* & Rosales MA*, Cubero-Font P, Calvo P, Álvarez R, Díaz-Espejo A, Colmenero-Flores JM (2019) Chloride as macronutrient increases water use efficiency by anatomically-driven reduced stomatal conductance and increased mesophyll diffusion to CO2. The Plant Journal 99:815-831. *Co-first authors.
  • Rosales MA, Maurel C, Nacry P (2019) Abscisic acid coordinates dose-dependent developmental and hydraulic responses of roots to water deficit. Plant Physiology 180:2198-2211.
  • Cubero-Font P*, Maierhofer T*, Jaslan J, Rosales MA, Espartero J, Díaz-Rueda P, Müller, HM, Hürter A-L, AL-Rasheid KAS, Marten I, Hedrich R, Colmenero-Flores JM, Geiger D (2016) Silent S-Type Anion Channel Subunit SLAH1 Gates SLAH3 Open for Chloride Root-to-Shoot Translocation. Current Biology 26:2213-2220.
  • Franco-Navarro JD, Brumós J, Rosales MA, Cubero-Font P, Talón, M, Colmenero-Flores JM (2016) Chloride regulates leaf cell size and water relations in tobacco plants. Journal of Experimental Botany 67:873-891.
  • Paneque M, De la Rosa JM, Franco-Navarro JD, Colmenero-Flores JM, Knicker H (2016) Effect of biochar amendment on morphology, productivity and water relations of sunflower plants under non-irrigation conditions. Catena 147:280-287

2011-2015

  • Pérez-Pérez JG, Gómez-Gómez AG, Botía P, Brumós J, Talón M, Colmenero-Flores JM (2015) Transcriptional profile analysis of young and mature leaves of citrus trees acclimated to salinity. Acta Horticulturae 1065:1359-1370.
  • Terol J, Carbonell J, Alonso R, Tadeo FR, Herrero-Ortega A, Ibañez V, Muñoz JV, López-García A, Estornell LH, Colmenero-Flores JM, et al. (2015) Sequencing of 150 citrus varieties: Linking genotypes to phenotypes. Acta Horticulturae 1065:585-590.
  • Colmenero-Flores JM & Rosales MA (2014) Interaction between salt and heat stress: when two wrongs make a right. Plant Cell Environment 37:1042–1045.
  • Aguado A, Capote N, Romero F, Dodd IC, Colmenero-Flores JM (2014) Physiological and gene expression responses of sunflower (Helianthus annuus L.) plants differ according to irrigation placement. Plant Science 227:37-44.
  • Rodríguez-Valentín R, Campos F, Battaglia M, Solórzano RM, Rosales MA, Covarrubias AA. (2014) Group 6 LEA proteins in monocotyledoneous plants: genomic organization and transcript accumulation patterns in response to stress in Oryza sativa. Plant Molecular Biology Reporter 32:198–208.
  • Allario T, Brumos J, Colmenero-Flores JM, Iglesias DJ, Pina JA, Navarro L, et al. (2013) Tetraploid Rangpur lime rootstock increases drought tolerance via enhanced constitutive root abscisic acid production. Plant Cell and Environment 36:856-868.
  • Rosales MA, Cuellar-Ortiz SM, Arrieta-Montiel MP, Acosta-Gallegos J, Covarrubias AA (2013) Physiological traits related to terminal drought resistance in common bean (Phaseolus vulgaris). Journal of the Science of Food & Agriculture 93:324-331.
  • Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Rosales MA, Blasco B, Rios JJ, Romero L, Blumwald E, Ruiz JM (2012) Ammonium formation and assimilation in PSARK::IPT tobacco transgenic plants under low N. Journal of Plant Physiology 169:157-162.
  • Rosales MA, Ocampo E, Rodríguez-Valentín R, Olvera-Carrillo Y, Acosta-Gallegos J, Covarrubias AA (2012) Physiological analysis of common bean (Phaseolus vulgaris) cultivars uncovers characteristics related to terminal drought resistance. Plant Physiology and Biochemistry 56:24-34.
  • Allario T, Brumos J, Colmenero-Flores JM, Tadeo F, Froelicher Y, Talon M, et al. (2011) Large changes in anatomy and physiology between diploid Rangpur lime (Citrus limonia) and its autotetraploid are not associated with large changes in leaf gene expression. Journal of Experimental Botany 62:2507-2519.
  • Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Rosales MA, Blasco B, Rios JJ, Romero L, Blumwald E, Ruiz JM (2011) Cytokinin-dependent Improvement in Transgenic PSARK::IPT Tobacco under N Deficiency. Journal of Agriculture and Food Chemistry 59:10491-10495.
  • Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Rosales MA, Blasco B, Rios JJ, Romero L, Blumwald E, Ruiz JM (2011) Effect of cytokinins on oxidative stress in tobacco plants under nitrogen deficiency. Environmental and Experimental Botany 72:167-173.
  • Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Ríos JJ, Blasco B, Rosales MA, Melgarejo R, Romero L, Ruiz JM (2011) Ammonia production and assimilation: its importance as mechanism tolerance during water deficit in tomato plants. Journal of Plant Physiology 168:816-823.
  • Talon M, Cercos M, Iglesias DJ, Colmenero-Flores JM, Ibáñez V, Brumos J, et al. (2011) Citrus genomics and breeding: Identification of candidate genes by the use of mutants and microarrays. Acta Horticulturae 892:19-26.
  • Rosales MA, Cervilla LM, Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Blasco B, Ríos JJ, Soriano T, Castilla N, Romero L, Ruiz JM (2011) The effect of environmental conditions on nutritional quality of cherry tomato fruits: evaluation of two experimental Mediterranean greenhouses. Journal of the Science of Food & Agriculture 91, 152-162.
  • Blasco B, Ríos JJ, Leyva R, Cervilla LM, Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Rosales MA, Ruiz JM, Romero L (2011) Does iodine biofortification affect oxidative metabolism in lettuce plants? Biological Trace Element Research 142:831-842.

2006-2010

  • Brumós J, Talón M, Bouhlal R, Colmenero-Flores JM (2010) Cl- homeostasis in includer and excluder citrus rootstocks: Transport mechanisms and identification of candidate genes. Plant Cell and Environment 33:2012-2027.
  • Ríos JJ, Blasco B, Cervilla LM, Rubio-Wilhelmi MM, Rosales MA, Sánchez-Rodríguez E, Romero L, Ruiz JM (2010) Nitrogen-use efficiency in relation to different forms and application rates of Se in lettuce plants. Journal of Plant Growth Regulation 29:164-170.
  • Ríos JJ, Blasco B, Rosales MA, Sánchez-Rodríguez, Leyva R, Cervilla LM, Romero L, Ruiz JM (2010) Response of nitrogen metabolism in lettuce plants subjected to different doses and forms of selenium. Journal of the Science of Food & Agriculture 90:1914-1919.
  • Blasco B, Ríos JJ, Cervilla LM, Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Rosales MA, Ruiz JM, Romero L (2010) Photorespiration process and nitrogen metabolism in lettuce plants (Lactuca sativa): induced changes in response to iodine biofortification. Journal of Plant Growth Regulation 29:477-486.
  • Gimeno J, Gadea J, Forment J, Pérez-Valle J, Santiago J, Martínez-Godoy MA, Colmenero-Flores JM, et al. (2009) Shared and novel molecular responses of mandarin to drought. Plant Molecular Biology 70:403-420.
  • Brumós J, Colmenero-Flores JM, Conesa A, Izquierdo P, Sánchez G, Iglesias DJ, et al. (2009) Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks. Functional Integrative Genomics 9:293-309.
  • Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Cervilla LM, Blasco B, Ríos JJ, Rosales MA, Romero L, Ruiz JM (2009) Genotypic differences in some physiological parameters symptomatic for oxidative stress under drought in tomato plants. Plant Science 178:30-40.
  • Cervilla LM, Blasco B, Ríos JJ, Rosales MA, Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Romero L, Ruiz JM (2009) Response of nitrogen metabolism to boron toxicity in tomato plants. Plant Biology 11:671-677.
  • Rosales MA, Cervilla LM, Ríos JJ, Blasco B, Sánchez-Rodríguez E, Romero L, Ruiz JM (2009) Environmental conditions affect pectin solubilization in cherry tomato fruits grown in two experimental Mediterranean greenhouses. Environmental & Experimental Botany 67:320-327.
  • Cervilla LM, Rosales MA, Rubio-Wilhelmi MM, Blasco B, Ríos JJ, Romero L, Ruiz JM (2009) Involvement of lignification and membrane permeability in the tomato root response to boron toxicity. Plant Science 176:545-552.
  • Rosales MA, Ríos JJ, Cervilla LM, Rubio-Wilhelmi MM, Blasco B, Ruiz JM, Romero L (2009) Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses. Journal of the Science of Food & Agriculture 89, 735-742.
  • Ríos JJ, Blasco B, Cervilla LM, Rosales MA, Sánchez-Rodríguez E, Romero L, Ruiz JM (2009) Production and detoxification of H2O2 in lettuce plants exposed to selenium. Annals of Applied Biology 154:107-116.
  • Tadeo FR, Cercós M, Colmenero-Flores JM, Iglesias DJ, Naranjo MA, Ríos G, et al. (2008) Molecular Physiology of Development and Quality of Citrus. Advances in Botanical Research 47:147-223.
  • López-Carrión AI, Castellano R, Rosales MA, Ruiz JM, Romero L (2008) Role of nitric oxide under saline stress: implications on proline metabolism. Biologia Plantarum 52:587-591.
  • Ríos JJ, Rosales MA, Blasco B, Cervilla LM, Romero L, Ruiz JM (2008) Biofortification of Se and induction of the antioxidant capacity in lettuce plants. Scientia Horticulturae 116:248-255.
  • Iglesias DJ, Cercós M, Colmenero-Flores JM, Naranjo MA, Ríos G, Carrera E, et al. (2007) Physiology of citrus fruiting. Brazilian Journal of Plant Physiology 19:333-362.
  • Rosales MA, Rubio-Wilhelmi MM, Castellano R, Castilla N, Ruiz JM, Romero L (2007) Sucrolytic activities in cherry tomato fruits in relation to temperature and solar radiation. Scientia Horticulturae 113:244-249.
  • Colmenero-Flores JM, Martínez G, Gamba G, Vázquez N, Iglesias DJ, Brumós J, et al. (2007) Identification and functional characterization of cation-chloride cotransporters in plants. Plant Journal 50:278-292.
  • Rosales MA, Rios JJ, Castellano R, López-Carrión AI, Romero L, Ruiz JM (2007) Proline metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of Horticultural Science & Biotechology 82:739-744.
  • Rosales MA, Ruiz JM, Hernández J, Soriano T, Castilla N, Romero L (2006) Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of the Science of Food & Agriculture 86:1545-1551.
  • Ruiz JM, Ríos JJ, Rosales MA, Rivero RM, Romero L (2006) Grafting between tobacco plants to enhance salinity tolerance. Journal of Plant Physiology 163:1229-1237.

1997-2005

  • Reyes JL, Rodrigo M, Colmenero-Flores JM, Gil J, Garay-Arroyo A, Campos F, et al. (2005) Hydrophilins from distant organisms can protect enzymatic activities from water limitation effects in vitro. Plant Cell and Environment 28:709-718.
  • Forment J, Gadea J, Huerta L, Abizanda L, Agusti J, Alamar S, Colmenero-Flores JM, et al. (2005) Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies. Plant Molecular Biology 57):375-391.
  • Campos-Álvarez F, Cruz-García F, Torres-Espinosa A, Sánchez-Jiménez M, Colmenero-Flores JM, Smith-Espinoza C, et al. (2002) Expression of late embryogenesis abundant (lea) protein codifying genes during osmopriming of maize and bean seeds. Agrociencia 36:461-470.
  • Garay-Arroyo A, Colmenero-Flores JM, Garciarrubio A, Covarrubias AA. (2000) Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. Journal of Biological Chemistry 275:5668-5674.
  • Colmenero-Flores JM, Moreno LP, Smith CE, Covarrubias AA. (1999) Pvlea-18, a member of a new late-embryogenesis-abundant protein family that accumulates during water stress and in the growing regions of well-irrigated bean seedlings. Plant Physiology 120:93-103.
  • Villanueva MA, Campos F, Díaz C, Colmenero-Flores JM, Dantán E, Sánchez F, et al. (1999) Actin expression in germinating seeds of Phaseolus vulgaris L. Planta 207:582-589.
  • Porta H, Rueda-Benítez P, Campos F, Colmenero-Flores JM, Colorado JM, Carmona MJ, et al. (1999) Analysis of lipoxygenase mRNA accumulation in the common bean (Phaseolus vulgaris L.) during development and under stress conditions. Plant Cell Physiology 40:850-858.
  • Folch-Mallol JL, Manyani H, Marroquí S, Sousa C, Vargas C, Nava N, Colmenero-Flores JM, et al. (1998) Sulfation of nod factors via nodHPQ is nodD independent in Rhizobium tropici CIAT899. Molecular Plant-Microbe Interactions 11:979-987.
  • Colmenero-Flores JM, Campos F, Garciarrubio A, Covarrubias AA. (1997) Characterization of Phaseolus vulgaris cDNA clones responsive to water deficit: Identification of a novel late embryogenesis abundant-like protein. Plant Molecular Biology 35:393-405.

2011-2020

  • Regulation of plant development and crop management through chloride nutrition: a novel tool to improve water- and nitrogen-use efficiency (ChlorPlant, H2020-895613). 2020-2023. (259.398,72 €). European Commission, H2020-MSCA-CAR-2019. PIs: Miguel A. Rosales Villegas & Antonio Díaz-Espejo.
  • Chloride homeostasis: New Funtions on Early Plant Development, Hydraulic Architecture, and Salinity Tolerance of Crops “CLORHOME” (RTI2018-094460-B-100). 2019-2021. Programa Estatal de I+D+i Orientado a los Retos de la Sociedad (MICINN). PI: José M. Colmenero-Flores.
  • RNAseq de la deficiencia a Cloruro: Verificación de Genes Candidatos (CSIC-2018-40E132). 2019. PI: José M. Colmenero-Flores.
  • Transcriptoma-RNAseq de la Deficiencia a Cloruro y Caracterización de Canales Aniónicos” (CSIC-2015-40E108). 2017-2019. PI: José M. Colmenero-Flores.
  • Homeostasis de Cloruro en Plantas: Resistencia a Sequía, Interacción con Nitrato y Caracterización Molecular, CLORHIDRIC” (AGL2015-71386-R). 2016-2018. PEICTI 2013-2016. PI: José M. Colmenero-Flores.
  • Plants in search of water: physiological and molecular interplay between root hydraulics and architecture during drought stress (DROUGHTROOT, H2020-657374). 2016-2018. (173.076 €). European Commission, H2020-MSCA-2014. IPs: Miguel A. Rosales Villegas & Christophe Maurel.
  • Portainjertos Silvestres para el Cultivo del Olivar, SILVOLIVE (Ref. 20160020006629). 2017. FEDER-MAPAMA. AYUDAS PARA LA CREACIÓN DE GRUPOS OPERATIVOS SUPRA-AUTONÓMICOS. Empresa coordinadora: Viveros Sevilla S.A. PI: José M. Colmenero-Flores.
  • Estudio de variedades silvestres para su uso como portainjertos de olivo (CSIC-2016-40E069). 2016-2017. PI: José M. Colmenero-Flores.
  • Caracterización Funcional de Canales de Cloruro / Nitrato (CSIC-2015-40E108). 2015-2016. PI: José M. Colmenero-Flores.
  • Hormonal regulations of aquaporins and root hydraulic architecture: their role in water deficit tolerance (AQUAPORINROOT-FP7-26719-389). 2014-2016. European Commission & INRA. PI: Miguel A. Rosales Villegas.
  • Generación e identificación de nuevas variedades para su uso como portainjertos en la mejora del cultivo intensivo del olivar (Ref. 20134R089). 2013-2015. Programa para el Desarrollo de Tecnologías Innovadoras para la Modernización y Valorización de la Explotación Agraria Andaluza mediante Compra Pública Pre-comercial. FEDER, MINECO. Coordinador: Ángel Caballero. PI Hito-6: José M. Colmenero-Flores.
  • Secuenciación, Genotipado y desarrollo de herramientas Genómicas para la mejora de los Cítricos (CITRUSEQ) (PSE-060000-2009-8). 2009-2011. Plan Nacional I+D+I 2009. Proyectos Singulares y Estratégicos. Coordinador: Manuel Talón Cubillo. PI IRNAS: José M. Colmenero-Flores.
  • Alteraciones biogeoquímicas mediadas por aves acuáticas en ecosistemas terrestres mediterráneos” (BIOGEOBIRD) (P09-RNM-4987). 2011-2013. Junta de Andalucía, Proyectos de Excelencia. PI: Luis V. García Fernández. Participante: José M. Colmenero-Flores.
  • Tolerancia al déficit hídrico en plantas basada en la regulación de la homeostasis del anión Cl- y el nivel de ploidía, CLOROPLOID (AGL2009-08339). 2010-2013. Plan Nacional I+D+I 2009. PI: José M. Colmenero-Flores.
  • Estudio funcional de algunas hidrofilinas vegetales y el impacto de su expresión heteróloga en la tolerancia a sequía (CONACYT-132258). 2010-2013. Consejo Nacional de Ciencia y Tecnología (México). PI: Alejandra Covarrubias Robles. Participant: Miguel A. Rosales Villegas.

2001-2010

  • Caracterización funcional de factores transcripcionales involucrados en la respuesta a déficit hídrico en frijol: análisis de su potencial como herramientas para conferir o seleccionar resistencia a sequia (CONACYT-83553). 2009-2010. Consejo Nacional de Ciencia y Tecnología (México). PI: Francisco Campos. Participant: Miguel A. Rosales Villegas.
  • Un nuevo mecanismo de tolerancia a déficit hídrico en plantas superiores: caracterización fisiológica y molecular (PIE-200840I222). 2008-2009. CSIC-I3. PI: José M. Colmenero-Flores.
  • Análisis genómico y fenotípico de la variabilidad genética de los cítricos en relación con la tolerancia frente a los estreses abióticos. 2007-2008. PNI+D+I, Ministerio de Educación y Ciencia. PI: Manuel Talón Cubillo. Participant: José M. Colmenero-Flores.
  • Citrus rootstock breeding for efficient water and nutrient use (FP6-2003-INCO-MPC-015453). 2006-2008. European Commission. PI: Patrick Ollitrault. Participant: José M. Colmenero-Flores.
  • Tolerancia a la salinidad y al déficit hídrico en cítricos: bases fisiológicas y moleculares y criterios agronómico (AGL2003-08502-C04-01). 2004-2006. Plan Nacional de I+D+I. Ministerio de Ciencia y Tecnología. PI: Manuel Talón Cubillo. Participant: José M. Colmenero-Flores.
  • Bac-ends sequencing for the physical mapping of the Citrus clementina nuclear genome. GENOSCOPE (Gobierno francés). PIs: Manuel Talón Cubillo (España) / Raphael Morillon (Francia). Participant: José M. Colmenero-Flores.
  • Análisis nutricional de seis especies de quercíneas de interés forestal en Andalucía y propuestas para su gestión. 2004-2007. Proyecto de Excelencia, Junta de Andalucía. PI: Luis Romero Monreal. Participant: Miguel A. Rosales Villegas.
  • Evaluación ambiental y mejoras de la calidad de la producción y eficiencia de uso de recursos del agrosistema invernaderos mediterráneos (I+D+I INIA RTA03-096-C5-1). 2003-2006. PI: Nicolás Castilla. Participant: Miguel A. Rosales.
  • Mejora de la tolerancia salina de cítricos potenciando la compartimentalización de NaCl en portainjertos” (BIO-2000-0174-P4-02). 2002-2004. Ministerio de Ciencia y Tecnología, Plan Nacional de I+D+I, CICyT-P4. PI (Viveros Sevilla S.A.): José M. Colmenero-Flores.
  • Obtención de portainjertos de cítricos resistentes a salinidad mediante transformación con un gen excluidor de sodio (FIT-010000-2001-134), 2001. Ministerio de Ciencia y Tecnología, Plan Nacional de I+D+I, PROFIT. PI: José M. Colmenero-Flores.
  • Producción de cítricos, olivos y frutales de hueso mediante cultivo in-vitro de tejidos vegetales. Desarrollo de técnicas moleculares de certificación varietal y control de la estabilidad genética” (FIT-010000-2001-78). 2001-2002. Ministerio de Ciencia y Tecnología, Plan Nacional de I+D+I, PROFIT. PI: José M. Colmenero-Flores.

1993-2000

  • Estudio de genes y proteínas involucradas en la respuesta a déficit hídrico en plantas superiores y levadura (26242N). 1998-2000. Consejo Nacional de Ciencia y Tecnología, México. PI: Alejandra Covarrubias Robles. Participant: José M. Colmenero-Flores.
  • El papel de las proteínas LEA en la tolerancia a la sequía en plantas. 1997-1999. Consejo Nacional de Ciencia y Tecnología (México) y KFA (Alemania). PIs: Alejandra Covarrubias Robles y Dorothea Bartles. Participant: José M. Colmenero-Flores.
  • Caracterización de genes involucrados por déficit de agua en Phaseolus vulgaris (0054N9106). 1994-1996. Consejo Nacional de Ciencia y Tecnología, México. PI: Alejandra Covarrubias Robles. Participant: José M. Colmenero-Flores.
  • Aislamiento y caracterización de genes inducidos en la respuesta a déficit hídrico en Phaseolus vulgaris (0131PN). 1993-1995. Consejo Nacional de Ciencia y Tecnología, México. PI: Alejandra Covarrubias Robles. Participant: José M. Colmenero-Flores.