Plant Biomass – Use and Valuation

Towards selective peroxygenation of plant lipids as new industrial raw materials in bio-intelligent manufacturing (OXYLIPIDS)
Subproject Title 2: Selective oxygenation for bio-based products
Funding Entity: MICINN (TED2021-129264B-C32)
Amount of the grant Subproject 2: 270,250 €.
Principal Researcher: Ana Gutiérrez
Duration: 2022 – 2024

Revalorization of potato peel by-products into sustainable multifunctional lacquers for metal food packaging (pop-up)
Funding Entity: MICINN – “Proyectos de Transición Ecológica y Transición Digital-2021” (TED2021-129656B-I00)
Grant amount: 108,100.00 €.
Principal researchers responsible: José A. Heredia-Guerrero (IHSM-CSIC), José J. Benítez Jiménez (ICMS-CSIC)
Principal Researcher IRNAS: José C. del Río/Jorge Rencoret
Duration: 2022 – 2024

Lignins and lipids: renewable sources of new bioproducts (LILÍ)
Funding entity: MICINN (PID2020-118968RB-I00)
Grant amount: 242.000 € (and predoctoral contract FPI)
Principal Researchers: Jorge Rencoret/Ana Gutiérrez
Duration: 2021 – 2024

Chemical structure and valorization of lignins from lignocellulosic residues of agricultural activity.
Funding Entity: Junta de Andalucía (Modalidad Retos de la sociedad andaluza, PY20_00017)
Amount of the grant: 85 000,00 €.
Principal Researcher: José Carlos del Río
Duration: 2021 – 2023

Oxidative enzymes for the Biorefineries of the Future
Funding Entity: CSIC (202040E185) (Intramural Project)
Grant amount: 181,037 €.
Principal researcher: Ana Gutiérrez
Duration: 2020 – 2023

Lignins from lignocellulosic residues of agricultural activity: Chemical structure and valorization (AGROLIGNIN)
Funding entity: MINECO (AGL2014-53730-R)
Grant amount: 141,570 €.
Principal researchers in charge: José C. del Río/Ana Gutiérrez
Duration: 2018 – 2020

Enzymes for lipid and lignin modification in lignocellulose Biorefineries
Funding Entity: CSIC (201740E071) (Intramural Project)
Grant amount: 134,504 €.
Principal researcher: Ana Gutiérrez
Duration: 2017 – 2020

Characterization and valorization of native and technical lignins for their improved industrial use
Funding entity: Funding entity or bodies: Spanish Ministry of Economy and Competitiveness (co-financed by FEDER funds). Name of the programme: National Programme for Research aimed at the Societal Challenges (Code according to the funding entity: CTQ2014-60764-JIN).
Participants: IRNAS-CSIC (Seville)
Grant amount: 202,070 €.
Principal Investigator: Jorge Rencoret
Duration: 2015 – 2018

Enzymatic modification of lignin and lipids in lignocellulose biorefineries (BIORENZYMERY)
Funding Entity: MINECO (AGL2014-53730-R)
Participants: IRNAS-CSIC (Seville)
Grant amount: 223,850 € (and predoctoral contract FPI)
Principal Investigator: Ana Gutiérrez
Other Researchers: José C. del Río
Duration: 2015 – 2017

Biotechnology for lignocellulose biorefining
Funding Entity: CSIC-Proyecto Intramural 201440E097
Participants: IRNAS-CSIC (Seville)
Grant amount: 180,000 €.
Principal Investigator: Ana Gutiérrez
Other Researchers: José C. del Río
Duration: 2014 – 2017

Development of optimized enzymatic pre-treatments for the deconstruction of lignocellulosic materials (LIGNOCELL)
Funding Entity: MICINN (AGL2011-25379)
Participants: IRNAS-CSIC (Seville)
Amount of the grant: 121,000 € (and FPI grant)
Principal Investigator: José C. del Río
Other IRNAS researchers: Ana Gutiérrez
Duration: 2012 – 2014

Fast-growing lignocellulosic crops: chemical composition and industrial utilization
Funding Entity: CSIC-Proyecto Intramural 2010-40E-075
Participants: IRNAS-CSIC (Seville)
Grant amount: 80.725 €.
Principal Investigator: José C. del Río
Other Researchers: Ana Gutiérrez
Duration: 2010 – 2013

Use of agricultural and forestry crops for the production of pulp: enzymatic treatments for the removal of lipids and lignin from pulp (ELLE).
Funding Entity: MICINN (AGL2008-00709)
Participants: IRNAS-CSIC (Seville)
Amount of the grant: 166,980 € (and FPI grant)
Principal researcher: Ana Gutiérrez
Other Researchers: José C. del Río
Duration: 2009 – 2011

Valorization of agroforestry products for pulp production: chemical characterization and structural modification of their constituents in the cooking and bleaching processes (AGROFOREST).
Funding Entity: MEC-National Program of Agrifood Resources and Technologies (AGL2005-01748)
Participants: IRNAS-CSIC (Seville)
Amount of the grant: 119,000 € (and FPI grant)
Principal Investigator: José C. del Río
Other Researchers: Ana Gutiérrez
Duration: 2005 – 2008

Chemical structure and biodegradability of lignin and extractable compounds in different agroforestry crops (LIGEX)
Funding Entity: MCyT (AGL2002-00393 AGR-FOR).
Participants: IRNAS-CSIC (Seville)
Amount of the grant: 127,270 €.
Principal researcher: Ana Gutiérrez
Other Researchers: José C. del Río
Duration: 2002 – 2005

Identification, biodegradability and elimination of the compounds responsible for the formation of pitch deposits in Eucalyptus globulus kraft pulps obtained by Cl2 free bleaching.
Funding Entity: CICYT/ FEDER Project 1FD97-0742.
Participants: ENCE (Pontevedra), CIB-CSIC (Madrid), IRNAS-CSIC (Sevilla)
Amount of the grant: 29,750,950 pts.
Principal Researcher IRNAS: José C. del Río
Other IRNAS researchers: Ana Gutiérrez
Duration: 1999 – 2001

Gómez de Santos P., González-Benjumea A., Fernández-García A., Aranda C., Wu Y., But A., Molina-Espeja P., Mate Maté D., González-Pérez D., Zhang W., Kiebist J., Scheibner K., Hofrichter M., Świderek K., Moliner V., Sanz-Aparicio J., Hollmann F., Gutiérrez A., Alcalde M. (2023) Engineering a highly regioselective fungal peroxygenase for the synthesis of hydroxy fatty acids. Angewandte Chemie International Edition 62: e202217372. (10.1002/anie.202217372)

Rencoret J., Marques G., Rosado M.J., Benito J., Barro F., Gutiérrez A., del Río J.C. (2023) Variations in the composition and structure of the lignins of oat (Avena sativa L.) straws according to variety and planting season, Int. J. Biol. Macromol. 242, 124811. (http://doi.org/10.1016/j.ijbiomac.2023.124811).

Kim H., Rencoret J., Elder T.J., del Río J.C., Ralph J. (2023) Biomimetic oxidative copolymerization of hydroxystilbenes and monolignols, Sci. Adv. 9, eade5519. (http://doi.org/10.1126/sciadv.ade5519).

Ralph J., Kim H., Lu F., Smith R.A., Karlen S.D., Nuoendagula, Yoshioka K., Eugene A., Liu S., Sener C., Ando D., Chen M., Li Y., Landucci L.L., Ralph S.A., Timokhin V.I., Lan W., Rencoret J., del Río J.C. (2023) Lignins and Lignification: New Developments and Emerging Concepts, in: Salminen J., Wähälä K., Freitas V., Quideau S. (Eds.), Recent Adv. Polyphenol Res., 1st ed., Wiley, 2023: pp. 1–50. (http://doi.org/10.1002/9781119844792.ch1).

Rodríguez-Escribano D., De Salas F., Pliego R., Marques G., Levée T., Suonpää A., Gutiérrez A., Martínez A.T., Ihalainen P., Rencoret J., Camarero S. (2023) Depolymerisation of kraft lignin by tailor-made alkaliphilic fungal laccases, Polymers. 15, 4433. (http://doi.org/10.3390/polym15224433).

Rosado M.J., Rencoret J., Gutiérrez A., del Río J.C. (2023) Structural characterization of the milled-wood lignin isolated from sweet orange tree (Citrus sinensis) pruning residue, Polymers. 15, 1840. (http://doi.org/10.3390/polym15081840).

Zhang W., Jiménez-Jiménez A., Capellades M., Rencoret J., Kashyap A., Coll N.S. Determination of de novo suberin-lignin ferulate deposition in xylem tissue upon vascular pathogen attack, in: J. Agusti (Ed.), Xylem, Springer US, New York, NY, 2024: pp. 117–127. (http://doi.org/10.1007/978-1-0716-3477-6_9).

Rencoret J., Rosado M.J., Kim H., Tymokhin V., Gutiérrez A., Bausch F., Rosenau T., Potthast A., Ralph J., and del Río J.C. (2022) Flavonoids naringenin chalcone, naringenin, dihydrotricin, and tricin are lignin monomers in papyrus. Plant Physiology 188, 208-219. (DOI: 10.1093/plphys/kiab469).

Elder T., del Río J.C., Ralph, J., Rencoret J., and Kim H. (2022) Density Functional Theory study on the coupling and reactions of diferuloylputrescine as a lignin monomer. Phytochemistry 197, 113122. (DOI: 10.1016/j.phytochem.2022.113122).

Rosado M.J., Marques G., Rencoret J., Gutiérrez A., Bausch F., Rosenau T., Potthast A., and del Río J.C.* (2022) Chemical composition of the lipophilic compounds from the rind and pith of papyrus (Cyperus papyrus L.) stems. Frontiers in Plant Science 13: 1097866. (DOI: 10.3389/fpls.2022.1097866).

Rosado M.J., Marques G., Rencoret J., Gutiérrez A., and del Río J.C. (2022) Chemical composition of lipophilic compounds from rice (Oryza sativa) straw – An attractive feedstock for obtaining valuable phytochemicals. Frontiers in Plant Science 13: 868319. (DOI: 10.3389/fpls.2022.868319).

Olmedo A., Ullrich R., Hofrichter M., del Río J.C., Martínez A.T., and Gutiérrez A. (2022) Novel fatty acid chain-shortening by fungal peroxygenases yielding 2C-shorter dicarboxylic acids. Antioxidants 11: 744. (DOI: 10.3390/antiox11040744)

Subramani V.B., Shi C., Moghaddam L., Atanda L., del Río J.C., Rencoret J., Bartley J., and Doherty W.O.S. (2022) Cotton farming sustainability: Formation of trans-isoeugenol/bio-aromatics, 5-chloromethylfurfural, C13-C17 liquid hydrocarbons & fertilizer from cotton gin trash. Journal of Cleaner Production 363: 132404 (DOI: 10.1016/j.jclepro.2022.132404).

Bausch F., Rosado M.J., Rencoret J., Marques G., Gutiérrez A., Graf J., del Río J.C., Rosenau T., and Potthast A. (2022) Papyrus production revisited: differences between ancient and modern production modes. Cellulose 29, 4931-4950. (DOI: 10.1007/s10570-022-04573-y).

Heinz O., Rencoret J., del Río J.C., and Ferraz A. (2022) Using undigested biomass solid leftovers from the saccharification process to integrate lignosulfonate production in a sugarcane bagasse biorefinery. ACS Sustainable Chemistry & Engineering 10, 7576-7585. (DOI: 10.1021/acssuschemeng.2c01274).

del Río J.C., Rencoret J., Gutiérrez A., Kim H., and Ralph J. (2022) Unconventional lignin monomers – Extension of the lignin paradigm. Advances in Botanical Research 104, 1-39. (DOI: 10.1016/bs.abr.2022.02.001).

Ferreira S.S., Goeminne G., Simões M.S., Pina A.V, de Lima L.G.A., Pezzard J., Gutiérrez A., Rencoret J., Mortimer J.C., del Río J.C., Boerjan W., and Cesarino I. (2022) Transcriptional and metabolic changes associated with internode development and reduced cinnamyl alcohol dehydrogenase activity in sorghum. Journal of Experimental Botany 73, 6307-6333. (DOI: 10.1093/jxb/erac300).

Loperena-Barber M., Khames M., Leclercq S.O., Zygmunt M.S., Babot E.D., Zúñiga-Ripa A., Gutiérrez A., Oumouna M., Moriyón I., Cloeckaert A., Álvarez R.C. (2022) Pseudochrobactrum algeriensis sp. nov., isolated from lymph nodes of Algerian cattle. International Journal of Systematic and Evolutionary Microbiology, 72 (2), 005223. (DOI: 10.1099/ijsem.0.005223)

Babot E.D., Aranda C., Kiebist J., Scheibner K., Ullrich R., Hofrichter M., Martínez A.T., Gutiérrez A. (2022) Enzymatic epoxidation of long-chain terminal alkenes by fungal peroxygenases. Antioxidants, 11 (3), 522. (DOI: 10.3390/antiox11030522)

Kashyap A., Jiménez-Jiménez A.L., Zhang W., Capellades M., Srinivasan S., Laromaine A., Serra O., Figueras M., Rencoret J., Gutiérrez A., Valls M., Coll N.S. (2022) Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato. New Phytologist, 234 (4): 1411-1429. (DOI: 10.1111/nph.17982)

Capellades M., Coll N.S., Figueras M., Gutiérrez A., Jiménez-Jiménez Á.L., Kashyap A., Laromaine A., Rencoret J., Serra O., Srinivasan S., Valls M., and Zhang W. (2022) Corrigendum to: Induced ligno-suberin vascular coating and tyramine-derived hydroxycinnamic acid amides restrict Ralstonia solanacearum colonization in resistant tomato. New Phytologist, 235 (6): 2496. (DOI: 10.1111/nph.18295)

Linde D., Santillana E., Fernández-Fueyo E., González-Benjumea A., Carro J., Gutiérrez A., Martínez A.T., Romero A. (2022) Structural characterization of two short unspecific peroxygenases: two different dimeric arrangements. Antioxidants, 11 (5), 891. (DOI: 10.3390/antiox11050891)

Linde D., González-Benjumea A., Aranda C., Carro J., Gutiérrez A., Martínez A.T. (2022) Engineering Collariella virescens peroxygenase for epoxides production from vegetable oil. Antioxidants, 11 (5), 915. (DOI: 10.3390/antiox11050915)

Carro, A. González-Benjumea, E. Fernández-Fueyo, C. Aranda, V. Guallar, A. Gutiérrez,and A.T. Martínez (2019) Modulating fatty acid epoxidation vs hydroxylation in a fungal peroxygenase. ACS Catalysis 9: 6234-6242. (DOI: 10.1021/acscatal.9b01454)

Ayuso-Fernández, J. Rencoret, A. Gutiérrez, F.J. Ruiz-Dueñas, and A.T. Martínez (2019) Peroxidase evolution in white-rot fungi follows wood lignin evolution in plants. PNAS. 116 (36): 17900-17905. (DOI: 10.1073/pnas.1905040116)

Lan, J. Rencoret, J.C. del Río, and J. Ralph (2019) Tricin in grass lignin: biosynthesis, characterization, and quantitation. In: Lignin: Biosynthesis, Functions and Economic Significance, Chapter 3, (Ed. F. Lu and F. Yue), Nova Science Publishers, Inc., New York, pp. 51-78. ISBN: 978-1-53614-769-8.

Rencoret, D. Neiva, G. Marques, A. Gutiérrez, H. Kim, J. Gominho, H. Pereira, J. Ralph, and J.C. del Río. (2019) Hydroxystilbene glucosides are incorporated into Norway spruce bark lignin. Plant Physiology 180, 1310-1321. (DOI: 10.1104/pp.19.00344). This paper was selected for a “News and Views” commentary; Annunziata M.G. What Is Lignin Made of? New Components Discovered! Plant Physiology 180, 1255.

Elder, JC. del Río, J. Ralph, J. Rencoret, H. Kim, and G.T. Beckham (2019) Radical coupling reactions of piceatannol and monolignols: a density functional theory study. Phytochemistry 164, 12–23. (DOI: 10.1016/j.phytochem.2019.04.003)

Aranda, M. Municoy, V. Guallar, J. Kiebist, K. Scheibner, R. Ullrich, J.C. del Río, M. Hofrichter, A.T. Martínez, and A. Gutiérrez (2019) Selective synthesis of 4-hydroxyisophorone and 4-ketoisophorone by fungal peroxygenases. Catalysis Science & Technology 9, 1398–1405. (DOI: 10.1039/c8cy02114g)

Penín, V. Santos, J.C. del Río, and J.C. Parajó (2019) Assessment on the chemical fractionation of Eucalyptus nitens wood: characterization of the products derived from the structural components. Bioresource Technology 281, 269–276. (DOI: 10.1016/j.biortech.2019.02.098)

Rencoret, A. Pereira, G. Marques, J.C. del Río, A.T. Martínez, and A. Gutiérrez (2019) A commercial laccase-mediator system to delignify and improve saccharification of the fast-growing Paulownia fortunei (Seem.) Hemsl. Holzforschung 73, 45–54. (DOI: 10.1515/hf-2018-0095)

Rencoret, A. Gutiérrez, E. Castro and J.C. del Río (2019) Structural characteristics of lignin in pruning residues of olive tree (Olea europeae L.). Holzforschung 73, 25–34. (DOI: 10.1515/hf-2018-0077)

J.J. Benítez, P.M. Castillo, J.C. del Río, M. León-Camacho, E. Domínguez, A. Heredia, S. Guzmán-Puyol, A. Athanassiou, and J.A. Heredia-Guerrero (2018) Valorization of tomato processing by-products: fatty acid extraction and production of bio-based materials. Materials 11, 2211.

Aranda, A. Olmedo, J. Kiebist, K. Scheibner, J.C. del Río, A.T. Martínez, and A. Gutiérrez (2018) Selective epoxidation of fatty acids and fatty acid methyl esters by fungal peroxygenases. ChemCatChem 10, 3964-3968.

Lan, F. Yue, J. Rencoret, J.C. del Río, W. Boerjan, F. Lu, J. Ralph (2018) Elucidating tricin-lignin structures: Assigning correlations in HSQC spectra of monocot lignins. Polymers 10:916.

Lesage-Meessen, M. Bou, C. Ginies, D. Chevret, D. Navarro, E. Drula, E. Bonnin, J.C. del Río, E. Odinot, A. Bisotto, J. Berrin, J. Sigoillot, C.B. Faulds, and A. Lomascolo (2018) Lavender and lavandin distilled straws: an untapped feedstock with great potential for the production of high-added value compounds and fungal enzymes. Biotechnology for Biofuels 11:217.

Reinoso, J. Rencoret, A. Gutiérrez, A.M.F. Milagres, J.C. del Río, and A. Ferraz (2018) Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse. Biotechnology for Biofuels 11:153.

Kaal, O. Serrano, J.C. del Río, J. Rencoret (2018) Radically different lignin composition in Posidonia species may link to differences in organic carbon sequestration capacity. Organic Geochemistry, 124: 247-256.

Ferraz, F. Reinoso, J. Rencoret, A. Gutiérrez, J.C. del Río, and A.M. Ferreira Milagres (2018) Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse. Biotechnology for Biofuels, 11:153.

Aranda, R. Ullrich, J. Kiebist, K. Scheibner, J.C. del Río, M. Hofrichter, A.T. Martínez and A. Gutiérrez (2018) Selective synthesis of the resveratrol analogue 4,4’-dihydroxy-trans-stilbene and stilbenoids modification by fungal peroxygenases. Catalysis Science & Technology, 8: 2394-2401.

J.C. del Río, J. Rencoret, A. Gutiérrez, H. Kim and J. Ralph (2018) Structural characterization of lignin from maize (Zea mays L.) fibers: Evidence for diferuloylputrescine incorporated into the lignin polymer in maize kernels. Journal of Agricultural and Food Chemistry, 66: 4402-4413.

Wanmolee, N. Laosiripojana, P. Daorattanachai, L. Moghaddam, J. Rencoret, J.C. del Río and W. Doherty (2018) Catalytic conversion of organosolv lignins to phenolic monomers in different organic solvents and the effect of operating conditions on yield with MIBK. ACS Sustainable Chemistry & Engineering 6 (3), 3010–3018.

Rencoret, H. Kim, A. Evaristo, A. Gutiérrez, J. Ralph and J.C. del Río (2018) Variability in Lignin Composition and Structure in Cell Walls of Different Parts of Macaúba (Acrocomia aculeata) Palm Fruit. Journal of Agricultural and Food Chemistry, 66: 138-153.

Kim, D. Padmakshan, Y. Li, J. Rencoret, R.D. Hatfield, J. Ralph. (2017) Characterization and elimination of undesirable protein residues in plant cell wall materials for enhancing lignin analysis by solution-state nuclear magnetic resonance spectroscopy. Biomacromolecules, 18: 4184-4195.

Sporck, F. Reinoso, J. Rencoret, A. Gutiérrez, J.C. del Río, A. Ferraz and A.M. Ferreira Milagres. (2017) Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches. Biotechnology for Biofuels, 10:296.

Olmedo, J.C. del Río, J. Kiebist, R. Ullrich, M. Hofrichter, K. Scheibner, A.T. Martínez and A. Gutiérrez. (2017) Fatty Acid Chain Shortening by a Fungal Peroxygenase. Chemistry-A European Journal, 23: 16985-16989.

Rencoret, A. Pereira, J.C. del Río, A.T. Martínez, A. Gutiérrez. (2017) Delignification and saccharification enhancement of sugarcane byproducts by a laccase-based pretreatment. ACS Sustainable Chemistry & Engineering, 5: 7145-7154.

van Kuijk, A. Sonnenberg, J. Baars, W. Hendriks, J.C. del Río, J. Rencoret, A. Gutiérrez, N.C.A. de Ruijter and J. Cone. (2017) Chemical changes and increased degradability of wheat straw and oak wood chips treated with the white rot fungi Ceriporiopsis subvermispora and Lentinula edodes. Biomass and Bioenergy, 105: 381-391.

A.T. Martínez, F.J. Ruiz-Dueñas, S. Camarero, A. Serrano, D. Linde, H. Lund, J. Vind, M. Tovborg, O.M. Herold-Majumdar, M. Hofrichter, C. Liers, R. Ullrich, K. Scheibner, G. Sannia, A. Piscitelli, C. Pezzella, M.E. Sener, S. Kılıç, W.J.H. van Berkel, V. Guallar, M.F. Lucas, R. Zuhse, R. Ludwig, F. Hollmann, E. Fernández-Fueyo, E. Record, C.B. Faulds, M. Tortajada, I. Winckelmann, J.-A. Rasmussen, M. Gelo-Pujic, A. Gutiérrez, J.C. del Río, J. Rencoret, M. Alcalde. (2017) Oxidoreductases on their way to industrial biotransformations. Biotechnology Advances, 35: 815-831.

J.C. del Río, J. Rencoret, A. Gutiérrez, H. Kim, J. Ralph. (2017) Hydroxystilbenes are monomers in palm fruit endocarp lignins. Plant Physiology, 174: 2072-2082.

Farias de Menezes, J. Rencoret, S.C. Nakanishi, V.M. Nascimento, V.F.N. Silva, A. Gutiérrez, J.C. del Río, G.J. de Moraes Rocha. (2017) Alkaline pretreatment severity leads to different lignin applications in sugar cane biorefineries. ACS Sustainable Chemistry & Engineering, 5: 5702-5712.

Moghaddam, J. Rencoret, V. R. Maliger, D.W. Rackemann, M.D. Harrison, A. Gutiérrez, J.C. del Río, W.O.S. Doherty. (2017) Structural Characteristics of bagasse furfural residue and its lignin component. A NMR, PyGC/MS, and FTIR Study. ACS Sustainable Chemistry & Engineering, 5: 4846-4855.

Pereira A., Hoeger I., Ferrer Carrera A., Rencoret J., del Río J.C., Kruus K., Rahikainen J., Kellock M., Gutiérrez A., Rojas O. (2017) Lignin films from spruce, eucalyptus and wheat straw studied with electroacoustic and optical sensors: Effect of composition and electrostatic screening on enzyme binding. Biomacromolecules, 18 (4): 1322-1332.

Shafrin F., Ferdous A.S., Sarkar S.K., Ahmed R., Amin A., Hossain K., Ashraf S., Sharker M., Rencoret J., Gutiérrez A., del Río, R.A., Sanan-Mishra N., Khan H. (2017) Modification of monolignol biosynthetic pathway in jute: Different gene, different cosequence. Scientific Reports (nature), 7: article 39984

Fornalé S., Rencoret J., García-Calvo L., Encina A., Rigau J., Gutiérrez A., del Río J.C., Caparrós-Ruiz D. (2017) Changes in cell wall polymers and degradability in maize mutants lacking 3’- and 5’-O-methyltransferases involved in lignin biosynthesis. Plant and Cell Physiology, 58 (2): 240-255.

Lan W., Rencoret J., Lu F., Karlen S.D., Smith B.G., Harris P.J., del Rio J.C., Ralph J. (2016) Tricin-lignins: occurrence and quantitation of tricin in relation to phylogeny. The Plant Journal, 88: 1046-1057.

Lan W., Morreel K., Lu F., Rencoret J., del Rio J.C., Voorend W., Vermerris W., Boerjan W., Ralph J. (2016) Maize Tricin-Oligolignol Metabolites and Their Implications for Monocot Lignification. Plant Physiology, 171: 810-820.

Lourenço A., Rencoret J., Chemetova C., Gutiérrez A., del Río J.C., Pereira H. (2016) Lignin composition and structure differs between xylem,phloem and phellem in Quercus suber L. Frontiers in Plant Science, 7: article 1612.

Olmedo A., Aranda C., del Río J.C., Kiebist J., Scheibner K., Martínez A.T., Gutiérrez A. (2016) From alkanes to carboxylic acids: Terminal oxygenation by a fungal peroxygenase. Angewandte Chemie International Edition, 55: 12248-12251.

Tana T., Zhang Z., Moghaddam L., Rackemann D., Rencoret J., Gutiérrez A., del Río J.C., Doherty W. (2016) Structural Changes of Sugarcane Bagasse Lignin during Cellulosic Ethanol Production Process. ACS Sustainable Chemistry & Engineering 4: 5483–5494.

Klamrassamee T., Tana T., Laosiripojana N., Moghaddam L., Zhang Z., Moghaddam L., Rackemann D., Rencoret J., Gutiérrez A., del Río J.C., Doherty W. (2016) Effects of an alkali-acid purification process on the characteristics of eucalyptus lignin fractionated from a MIBK-based organosolv process. RSC Advances, 6: 92638

Sáez-Jiménez V., Rencoret J., Rodríguez-Carvajal M.J., Gutiérrez A., Ruiz-Dueñas F.J., Martínez A.T. (2016) Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin. Biotechnology for Biofuels, 9:198.

Rencoret J., del Río J.C, Nierop K.G.J., Gutiérrez A., Ralph J. (2016) Rapid Py-GC/MS assessment of the structural alterations of lignins in genetically modified plants. Journal of Analytical and Applied Pyrolysis, 121: 155-164.

Van Kuijk S., del Río J.C., Rencoret J., Gutiérrez A., Sonnenberg A., Baars J., Hendriks W., Cone J. (2016) Selective ligninolysis of wheat straw and wood chips by the white-rot fungus Lentinula edodes and its influence on in vitro rumen degradability. Journal of Animal Science and Biotechnology, 7:55.

Rencoret J., Pereira A., del Río J.C., Martínez A.T., Gutiérrez A. (2016) Laccase-mediator pretreatment of wheat straw degrades lignin and improves saccharification. Bioenergy Research, 9: 917-930.

Rodríguez-Celma J., Lattanzio G., Villarroya D., Gutiérrez-Carbonell E., Ceballos-Laita L., Rencoret J., Gutiérrez A., del Río J.C, Grusak M.A, Abadía A., Abadía J., López-Millán A.F. (2016) Effects of Fe deficiency on the protein profiles and lignin composition of stem tissues from Medicago truncatula in absence or presence of calcium carbonate. Journal of Proteomics, 140: 1-12.

Fernández-Fueyo E., Ruiz-Dueñas F.J., López-Lucendo M.F., Pérez-Boada M., Rencoret J., Gutiérrez A., Pisabarro A.G., Ramírez L., Martínez A.T. (2016) A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus. Biotechnology for Biofuels, 9:49.

Del Río J.C., Evaristo A.B., Marques G., Martín-Ramos P., Martín-Gil J., Gutiérrez A. (2016) Chemical composition and thermal behavior of the pulp and kernel oils from macauba palm (Acrocomia aculeata) fruit. Industrial Crops and Products, 84: 294-304.

Del Río J.C., Prinsen P., Cadena E.M., Martínez A.T., Gutiérrez A., Rencoret J. (2016) Lignin-carbohydrate complexes from sisal (Agave sisalana) and abaca (Musa textilis): chemcal composition and structural modifications during the isolation process. Planta, 243: 1143-1158.

Lucas F., Babot E.D., Cañellas M., del Río J.C., Kalum L., Ullrich R., Hofrichter M., Guallar V., Martínez A.T., Gutiérrez A. (2016) Molecular determinants for selective C₂₅-hydroxylation of vitamins D₂ and D₃ by fungal peroxygenases Catalysis Science & Technology, 6: 288-295.

Velez Marques A., Rencoret J., Gutiérrez A., del Río J.C., Pereira H (2016) Ferulates and lignin structural composition in cork. Holzforschung, 70: 275-289.

Lan W., Lu F., Regner M., Zhu Y., Rencoret J., Ralph S.A., Zakai U.I., Morreel K., Boerjan W., Ralph J. (2015) Tricin, a flavonoid monomer in monocot lignification. Plant Physiology, 167: 1284-1295.

Lourenço A., Rencoret J., Chemetova C., Gominho J., Gutiérrez A., Pereira H., del Río J.C. (2015) Isolation and structural characterization of lignin from cardoon (Cynara cardunculus L.) stalks. Bioenergy Research, 8: 1946-1955.

Del Río J.C., Marques G., Lino A.G., Lima C.F., Colodette J., Gutiérrez A. (2015) Lipophilic phytochemicals from sugarcane bagasse and straw. Industrial Crops and Products, 77: 992-1000.

Sáez-Jiménez V., Baratto M.C., Pogni R., Rencoret J., Gutiérrez A., Santos J.I., Martínez A.T., Ruiz-Dueñas F.J. (2015) Demonstration of lignin-to-peroxidase direct electron transfer: A transient-state kinetics, directed mutagenesis, EPR and NMR study. The Journal of Biological Chemistry, 290 (38): 23201-23213.

Carro J., Ferreira P., Rodríguez L., Prieto A., Serrano A., Balcells B., Arda A., Jiménez-Barbero J., Gutiérrez A., Ullrich R., Hofrichter M., Martínez A.T. (2015) 5-Hydroxymethylfurfural conversion by fungal aryl-alcohol oxidase and unspecific peroxygenase. The FEBS Journal, 282: 3218-3229.

Del Río J.C., Lino A.G., Colodette J.L., Lima C.E., Gutiérrez A., Martínez A.T., Lu F., Ralph J., J.Rencoret (2015) Differences in the chemical structure of the lignins from sugarcane bagasse and straw. Biomass & Bioenergy, 81: 322-338

Fornalé S., Rencoret J., García-Calvo L., Capellades M., Encina A., Santiago R., Rigau J., Gutiérrez A., del Río J.C, Caparrós-Ruiz D. (2015) Cell wall modifications triggered by the down-regulation of coumarate 3-hydroxylase-1 in maize. Plant Science, 236: 272-282.

Babot E.D., del Río J.C., Cañellas M., Sancho F., Lucas F., Guallar V., Kalum L., Lund H., Gröbe G., Scheibner K., Ullrich R., Hofrichter M., Martínez A.T., Gutiérrez A. (2015) Regioselective oxyfunctionalization of steroids by fungal peroxygenases. Applied and Environmental Microbiology, 81(12): 4130-4142.

Rencoret J., Prinsen P., Gutiérrez A., Martínez A.T., del Río J.C. (2015) Isolation and structural characterization of the milled wood lignin, dioxane lignin and cellulolytic lignin preparations from brewer´s spent grain. Journal of Agricultural and Food Chemistry, 63: 603-613.

Babot E.D., del Río J.C., Kalum L, Martínez A.T., Gutiérrez A. (2015) Efficient and regioselective hydroxylation in the enzymatic production of 25-hydroxyvitamin D. ChemCatChem, 7: 283-290

Rico A., Rencoret J., del Río J.C., Martínez A.T., Gutiérrez A. (2015) In-depth 2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with laccase and mediators. Bioenergy Research, 8: 211-230.

Wilkerson C.G., Mansfield S.D., Lu F., Withers S., Park J.Y., Karlen S.D., Gonzales-Vigil E., Padmakshan D., Unda F., Rencoret J., Ralph J. (2014) Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone. Science, 344: 90-93.

Hori C., Ishida T., Igarashi K., Samejima M., Suzuki S., Master E., Ferreira P., Ruiz-Dueñas F.J., Held B, Canessa P., Larrondo L.F., Schmoll M., Druzhinina I.S., Kubicek P.C., Gaskell J., Kersten P., St. John F., Glasner J., Sabat G., Splinter BonDurant S., Khajamohiddin S., Yadav J., A.C. Mgbeahuruike, Kovalchuk A., Asiegbu F.O., Lackner G, Hoffmeister D., Rencoret J., Gutiérrez A., Sun H., Lindquist E., Barry K., Riley R., Grigoriev I.V., Henrissat B., Kües U., Berka R.M., Martínez A.T., Covert S.F., Blanchette R.A., Cullen D. (2014) Analysis of the Phlebiopsis gigantea genome, transcriptome and secretome gives insight into its pioneer colonization strategies of wood. PLOS Genetics, 10 (12): e1004759.

Pérez-Boada M., Prieto A., Prinsen P., Forquin-Gomez M.P., del Río J.C., Gutiérrez A., Martínez A.T., Faulds C.B. (2014) Enzymatic degradation of Elephant grass (Pennisetum purpureum) stems: Influence of the pith and bark in the total hydrolysis. Bioresource Technology, 167:469-475.

Martínez A.T., Ruiz-Dueñas F.J., Gutiérrez A., del Río J.C., Alcalde M., Liers M., Ullrich R., Hofrichter M., Scheibner K., Kalum L., Vind J., Lund H. (2014) Search, engineering and applications of new oxidative biocatalysts. Biofuels, Bioproducts & Biorefining, 8: 819-835.

Du X., Pérez-Boada M., Fernández C., Rencoret J., del Río J.C., Jiménez Barbero J., Li J., Gutiérrez A., Martínez A.T. (2014) Analysis of lignin-carbohydrate and lignin-lignin linkages after hydrolase treatment of xylan-lignin, glucomannan lignin and glucan-lignin complexes from spruce wood. Planta, 239:1079-1090.

Prinsen P., Gutiérrez A., Faulds C.B., del Río J.C. (2014) A comprehensive study of valuable lipophilic phytochemicals in wheat bran. Journal of Agricultural and Food Chemistry, 62:1664-1673.

Rencoret J., Aracri E., Gutiérrez A., del Río J.C., Torres A.L., Vidal T., Martínez A.T. (2014) Structural insights on laccase biografting of ferulic acid onto lignocellulosic fibres. Biochemical Engineering Journal, 86: 16-23.

Rico A., Rencoret J., del Río J.C., Martínez A.T., Gutiérrez A. (2014) Pretreatment with laccase and a phenolic mediator degrades lignin and enhances saccharification of Eucalyptus feedstock. Biotechnology for Biofuels, 7:6.

Prinsen P., Rencoret J., Gutiérrez A., Liitia T., Tamminen T., Colodette J.L., Berbis M., Jiménez-Barbero J., Martínez A.T., del Río J.C. (2013) Modification of the lignin structure during alkaline delignification of Eucalyptus wood by kraft, soda-AQ and soda-O₂ cooking. Industrial and Engineering Chemistry Research, 52: 15702-15712.

Du X., Li J., Gellerstedt G., Rencoret J., del Río J.C., A.T. Martínez, Gutiérrez A. (2013) Understanding pulp delignification by laccase-mediator systems through isolation and characterization of lignin-carbohydrate complexes. Biomacromolecules, 14: 3073-3080.

Babot E.D., del Río J.C., Kalum L., Martínez A.T., Gutiérrez A. (2013) Oxyfunctionalization of aliphatic compounds by a recombinant peroxygenase from Coprinopsis cinerea. Biotechnology and Bioengineering, 110(9): 2323-2332.

Del Río J.C., Prinsen P., Gutiérrez A. (2013) Chemical composition of lipids in brewer´s spent grain: A promising source of valuable phytochemicals. Journal of Cereal Science, 58: 248-254

Rencoret J., Marques G., Gutiérrez A., Jiménez-Barbero J., Martínez A.T., del Río J.C. (2013) Structural modifications of residual lignins from sisal and flax pulps during soda-AQ pulping and TCF/ECF bleaching. Industrial and Engineering Chemistry Research, 52: 4695-4703.

Rencoret J., Ralph J., Marques G., Gutiérrez A., Martínez A.T., del Río J.C. (2013) Structural characterization of lignin isolated from coconut (Cocos nucifera) coir fibers. Journal of Agricultural and Food Chemistry, 61: 2434-2445.

Del Río J.C., Prinsen P., Gutiérrez A. (2013) A comprehensive characterization of lipids in wheat straw. Journal of Agricultural and Food Chemistry, 61:1904-1913.

Zhang K., Bhuiya M.W., Rencoret J., Miao Y., Kim H., Ralph J., Liu C.J. (2012) An Engineered Monolignol 4-O-Methyltransferase Depresses Lignin Biosynthesis and Confers Novel Metabolic Capability in Arabidopsis. The Plant Cell Online, 24: 3135-3152.

Gutiérrez A., Rencoret J., Cadena E.M., Rico A., Barth D., del Río J.C., Martínez A.T. (2012) Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks. Bioresource Technology, 119: 114-122.

Prinsen P., Gutiérrez A., Del Río J.C. (2012) Lipophilic extractives from the cortex and pith of elephant grass (Pennisetum purpureum Schumach.) stems. Journal of Agricultural and Food Chemistry, 60: 6408-6417.

Del Río J.C., Rencoret J., Prinsen P., Martínez A.T., Ralph J., Gutiérrez A. (2012) Structural characterization of wheat straw lignin as revealed by analytical pyrolysis, 2D-NMR and reductive cleavage methods. Journal of Agricultural and Food Chemistry, 60: 5922-5935.

Del Río J.C., Prinsen P., Rencoret J., Nieto L., Jiménez-Barbero J., Ralph J., Martínez A.T., Gutiérrez A. (2012) Structural characterization of the lignin in the cortex and pith of Elephant grass (Pennisetum purpureum) stems. Journal of Agricultural and Food Chemistry, 60: 3619-3634.

Palacio-Chaves L., Zúñiga-Ripa A., Gutiérrez A., Gil-Ramírez Y., Conde-Álvarez R., Moriyón I., Iriarte M. (2012) Identification and functional analysis of the cyclopropane fatty acid synthase of Brucella abortus. Microbiology, 158: 1037-1044.

Prinsen P., Gutiérrez A., Rencoret J., Nieto L., Jiménez-Barbero J., Burnet A., Petit-Conil M., Colodette J., Martínez A.T., del Río J.C. (2012) Morphological characteristics and composition of lipophilic extractives and lignin in Brazilian woods from different eucalypt hybrids. Industrial Crops and Products, 36: 572-583.

Rencoret J., del Río J.C., Gutiérrez A., Martínez A.T., Li S., Parkås J., Lundquist K. (2012) Origin of the acetylated structures present in white birch (Betula pendula Roth) milled wood lignin. Wood Science and Technology, 46: 459-471.

Del Río J.C., Rencoret J., Gutiérrez A., Nieto L., Jiménez-Barbero J., Martínez A.T. (2011) Structural characterization of guaiacyl-rich lignins in flax (Linum usitatissimum) fibers and shives. Journal of Agricultural and Food Chemistry, 59:11088-11099.

Gutiérrez A., Babot E.D., Ullrich R., Hofrichter M., Martínez A.T., del Río J.C. (2011) Regioselective oxygenation of fatty acids, fatty alcohols and other aliphatic compounds by a basidiomycete heme-thiolate peroxidase. Archives of Biochemistry and Biophysics, 514: 33-43.

Babot E.D., Rico A., Rencoret J., Kalum L., Lund H., Romero J., del Río J.C., Martínez A.T., Gutiérrez A. (2011) Towards industrially-feasible delignification and pitch removal by treating paper pulp with Myceliophthora thermophila laccase and a phenolic mediator. Bioresource Technology, 102: 6717-6722.

Marques G., Molina S., Babot E.D., LundH., del Río J.C., Gutiérrez A. (2011) Exploring the potential of fungal manganese-containing lipoxygenase for pitch control and pulp delignification. Bioresource Technology, 102: 1338-1343.

Rencoret J., Gutiérrez A., Nieto L., Jiménez-Barbero J., Faulds C.B., Kim H., Ralph J., Martínez A.T., del Río J.C. (2011) Lignin composition and structure in young versus adult Eucalyptus globulus plants. Plant Physiology, 155: 667-682.

Martínez A.T., Rencoret J., Nieto L., Jiménez-Barbero J., Gutiérrez A., del Río J.C. (2011) Selective lignin and polysaccharide removal in natural fungal decay of wood as evidenced by in situ structural analysis. Environmental Microbiology, 13(1): 96-107.

Vanholme R., Ralph J., Akiyama T., Lu F., Rencoret J., Christensen J., Rohde A., Morreel K., DeRycke R., Kim H., Van Reusel B., Boerjan W. (2010) Engineering traditional monolignols out of lignins by concomitant up-regulation F5H1 and down-regulation of COMT in Arabidopsis. The Plant Journal, 64: 885-897

Aracri E., Fillat A., Colom J.F., Gutiérrez A., del Río J.C., Martínez A.T., Vidal T. (2010) Enzymatic grafting of simple phenols on flax and sisal pulp fibres using laccases. Bioresource Technology, 101: 8211-8216.

Marques G., Gutiérrez A., del Río J.C., Evtuguin D.V. (2010) Acetylated  heteroxylan from Agave sisalana and its behavior in alkaline pulping and TCF/ECF bleaching. Carbohydrate Polymers, 81: 517-523.

Marques G., Gamelas J.A.F., Ruiz-Dueñas J.F., del Río J.C., Evtuguin D.V., Martínez A.T., Gutiérrez A. (2010) Delignification of eucalypt kraft pulp with manganese-substituted polyoxometalate assisted by fungal versatile peroxidase. Bioresource Technology, 101: 5935-5940.

Prasetyo E.N., Kudanga T., Rencoret J., Gutiérrez A., del Río J.C., Santos J.I., Nieto L., Jiménez-Barbero, Martínez A.T., Li J., Gellerstedt G., Lepifre S., Silva C., Cavaco-Paulo A., Klausen B.S., Lutnaes B.F., NyanhongoG.S., Guebitz M. (2010) Polymerisation of lignosulfonates by the laccase-HBT(1-hydroxybenzotriazole) system improves dispersibility. Bioresource Technology, 101: 5054-5062.

Ferreira P., Hernández-Ortega A., Herguedas B., Rencoret J., Gutiérrez A., Martínez M.J., Jiménez-Barbero J., Medina M., Martínez A.T. (2010) Kinetic and chemical characterization of aldehyde oxidation by fungal aryl-alcohol oxidase. Biochemical Journal, 425: 585-593.

Marques G., del Río J.C. and Gutiérrez A. (2010) Lipophilic extractives from several nonwoody lignocellulosic crops (flax, hemp, sisal, abaca) and their fate during alkaline pulping and TCF/ECF bleaching. Bioresource Technology, 101: 260-267.