Group

Plant Ion and Water Regulation

RIH

Description

Climate change threatens crop production, necessary to feed a continuously growing population. Faced with adverse environmental conditions, increasing knowledge about the homeostatic regulation of mineral nutrients is necessary to: enhance crop growth and production; improve the nutritional value of foods; reduce the adverse effects of key environmental factors threatening agriculture, particularly water deficit and salinity. The Group, led by Dr. José M. Colmenero-Flores, develops several lines of research in this field.

1. Chloride as a Beneficial Macronutrient Function (Responsible, Dr. José M. Colmenero-Flores) We study chloride (Cl⁻) nutrition and its function in higher plants. Chloride has traditionally been considered harmful to crops due to its toxicity under saline conditions and its detrimental effect on nitrate (NO₃⁻) nutrition. However, our results have classified Cl⁻ as a beneficial macronutrient, due to its role in plant development and its enhancement of water, nitrogen, and CO₂ use efficiency, fundamental components of plant nutrition. Ensuring adequate Cl⁻ nutrition allows: reducing the amount of NO₃⁻ supplied as fertilizer to crops and its adverse environmental effects (e.g., pollution of the Mar Menor, Murcia); reducing the NO₃⁻ content in vegetables, decreasing their toxicity (can cause methemoglobinemia and gastric cancer); increasing resistance to drought and other adverse environmental factors.

2. Regulatory Components of Cl⁻ Homeostasis (Responsible, Dr. José M. Colmenero-Flores)

The homeostatic control of Cl⁻ is meticulously regulated by plants through environmental and developmental factors, and is coordinated with the transport and accumulation of NO₃⁻. We are identifying and characterizing the molecular mechanisms regulating these processes, including anionic transporters from the CCC, SLAC/SLAH, NPF, and NRT2 families, involved in Cl− and NO₃⁻ homeostasis.

3. Characterization of Wild Germplasm for Olive Crop Improvement (Responsible, Dr. José M. Colmenero-Flores)

We study the genotypic and phenotypic variability of wild olive genotypes (SILVOLIVE Collection), their importance for improving crop resistance and productivity, as well as their relevance for mitigating the effects of climate change. We collaborate closely and transfer results to companies in the agri-food and nursery sectors.

4. Epigenetic Tools in the Regulation of Ionic and Nutritional Homeostasis (Responsible, Dr. Fernando Alemán)

Potassium plays crucial roles in plants and is one of the nutrients that best participates in the plant acclimatization to water deficit. In the laboratory, we aim to achieve more efficient nutrient and water uptake in crops, crops better adapted to climate change, and sustainable agriculture. To achieve this, we are developing multidisciplinary approaches in the field of epigenetics and ABA-mediated cellular signaling, using structural biology tools and the CRISPR/dCas9 system.

Members of the research group

Dr. José Manuel Colmenero Flores

Científico Titular CSIC

Responsable del grupo

chemacf[AT]irnase.csic.es

Francisco J. Durán Gutiérrez

Técnico I+D+i

fj.duran[AT]csic.es

Marta Lucas Gutiérrez

Doctoranda

mlucas[AT]irnas.csic.es

Dr. Fernando Alemán

Científico Titular CSIC

Pablo Díaz Rueda

Doctorando

pdiaz[AT]irnas.csic.es

David Romero Jiménez

Contratado Pre-doctoral FPI

david.romero.j[AT]csic.es

Dra. Mª del Rosario Álvarez Morales

Profesora Colaboradora de la US

mrosario[AT]us.es

Procopio Peinado Torrubia

Doctorando

ppeinado[AT]irnas.csic.es

Former Members of the Research Group

Mª Ángeles Vargas Pérez. Bióloga. Máster en Biología Avanzada.
Blanca Beas Santos. Bióloga. Máster en Biología Avanzada.
Adrián Gómez Garzón (TFM). Biólogo. Máster en Biología Avanzada.
Dra. Paloma Cubero Font (Doctorado). Postdoc en BPMP-CNRS (Montpellier, Francia). Email: paloma.cubero-font[AT]supagro.fr
Dr. José Barrera Gavira (TFM). Investigador en University of Aberdeen (Aberdeen, Scotland). Email: jomasims[AT]hotmail.com
Alberto Vázquez Rodríguez (TFM). Biólogo, Informático y Desarrollador Android. Email: albvazrod[AT]gmail.com
Ana Angulo Fornos (TFM). Asesora técnico-sanitaria. Email: anagunos[AT]gmail.com
Gonzalo Lora Baranco (TFG). Consultor I+D. Email: gonzalolorabaranco93[AT]hotmail.com
Sofía Luque González (Contrato Técnico). Profesora de Química y Biología en Secundaria. Email: sofy_lg[AT]hotmail.com
Bernardino J. Sañudo Franquelo (Prácticas de Investigación). Biólogo. Urban sketcher. Ilustrador. Email: bernardinojsf[AT]gmail.com
Yurema Pinto González (Prácticas de Investigación). Técnico de Laboratorio Clínico y Biomédico. Email: yuremapintogonzalez[AT]gmail.com
Irene Robles Caballero (Prácticas de Investigación). Profesora. Email: irenerobles94[AT]hotmail.com
Claudia Ahumada García (Prácticas de Investigación). Profesora de secundaria. Email: claahugar[AT]gmail.com
Dr. Miguel A. Rosales Villegas. Científico titular (EEZ-CSIC)
Dr. Juan D. Franco Navarro. Doctor en Biología y Premio 25° Fertiberia. Investigador colaborador del Grupo de Regulación Iónica e Hídrica en Plantas (RIH) – IRNAS-CSIC, y técnico de calidad e integración I+D+i en CLECE Hospital Universitario de Puerto Real y Hospital de Alta Resolución de La Janda (Cádiz). Colegiado #4159 en el Colegio Oficial de Biólogos de Andalucía. Email: juandediosfn[AT]bioscriptsdb.com juandediosfranconavarro1982[AT]gmail.com
Francisco J. Moreno Racero. Dorctorando del IG-CSIC
Álvaro F. García Rodríguez. Doctorando del IG-CSIC
Ana Pérez Álvarez. Profesora de Secundaria

Members of the Plant Crop Biotechnology Service

Miriam Pérez Sayago

Responsable - Titulada Media

sayago[AT]irnase.csic.es

Antonio Salas Illescas

Graduado en Bioquímica

Máster en Biotecnología Industrial y agroalimentaria

antonio.salas[AT]csic.es

Projects

Characterization of Chloride Homeostasis in Plants: Interaction with Nitrate, Regulatory Mechanisms and Agronomic Applications, AGRICHLOR (PID2021-125157OB-I00). PEICTI 2021-2023 MICINN. Period: 2022-2025. Amount: €169,400. IP: José M. Colmenero Flores
Caracterización funcional de transportadores aniónicos de plantas mediante el uso de las plataformas de electrofisiología y de edición genómica Estancia del Dr. Colmenero en el Laboratorio “Plant Transport and Signalling” del Waite Research Institute, Universidad de Adelaida, Australia (Ref: PRX22/00615). Programa de Estancias de movilidad de investigadores senior en centros de investigación extranjeros (14.645,00 €) Ministerio de Universidades. IP: José M Colmenero Flores
SILVOLIVE, a Germplasm Collection of Wild Olive Genotypes for Olive Breeding” (Gen4Olive, UE-2020 Nº 101000427)European Comission 2023 (37,500 €). Empresa Coordinadora: Viveros Sevilla SA. IP: José M. Colmenero Flores.

Regulation of plant development and crop management through chloride nutrition: a novel tool to improve water- and nitrogen-use efficiency (ChlorPlant, H2020-895613). 2020-2023. (259.398,72 €). European Commission, H2020-MSCA-CAR-2019. IP: Miguel A. Rosales.
Chloride homeostasis: New Funtions on Early Plant Development, Hydraulic Architecture, and Salinity Tolerance of Crops “CLORHOME” (RTI2018-094460-B-100). 2019-2021. Programa Estatal de I+D+i Orientado a los Retos de la Sociedad (MICINN). IP: José M. Colmenero-Flores.
RNAseq de la deficiencia a Cloruro: Verificación de Genes Candidatos (CSIC-2018-40E132). 2019. IP: José M. Colmenero-Flores.
Transcriptoma-RNAseq de la Deficiencia a Cloruro y Caracterización de Canales Aniónicos” (CSIC-2015-40E108). 2017-2019. IP: José M. Colmenero-Flores.
Homeostasis de Cloruro en Plantas: Resistencia a Sequía, Interacción con Nitrato y Caracterización Molecular, CLORHIDRIC” (AGL2015-71386-R). 2016-2018. PEICTI 2013-2016. IP: José M. Colmenero-Flores.
Plants in search of water: physiological and molecular interplay between root hydraulics and architecture during drought stress (DROUGHTROOT, H2020-657374). 2016-2018. (173.076 €). European Commission, H2020-MSCA-2014. IPs: Miguel A. Rosales Villegas y Christophe Maurel.
Portainjertos Silvestres para el Cultivo del Olivar, SILVOLIVE (Ref. 20160020006629). 2017. FEDER-MAPAMA. AYUDAS PARA LA CREACIÓN DE GRUPOS OPERATIVOS SUPRA-AUTONÓMICOS. Empresa coordinadora: Viveros Sevilla S.A. IP: José M. Colmenero-Flores.
Estudio de variedades silvestres para su uso como portainjertos de olivo (CSIC-2016-40E069). 2016-2017. IP: José M. Colmenero-Flores.
Caracterización Funcional de Canales de Cloruro / Nitrato (CSIC-2015-40E108). 2015-2016. IP: José M. Colmenero-Flores.
Hormonal regulations of aquaporins and root hydraulic architecture: their role in water deficit tolerance (AQUAPORINROOT-FP7-26719-389). 2014-2016. European Commission & INRA. IP: Miguel A. Rosales Villegas.
Generación e identificación de nuevas variedades para su uso como portainjertos en la mejora del cultivo intensivo del olivar (Ref. 20134R089). 2013-2015. Programa para el Desarrollo de Tecnologías Innovadoras para la Modernización y Valorización de la Explotación Agraria Andaluza mediante Compra Pública Pre-comercial. FEDER, MINECO. Coordinador: Ángel Caballero. IP Hito-6: José M. Colmenero-Flores.
Secuenciación, Genotipado y desarrollo de herramientas Genómicas para la mejora de los Cítricos (CITRUSEQ) (PSE-060000-2009-8). 2009-2011. Plan Nacional I+D+I 2009. Proyectos Singulares y Estratégicos. Coordinador: Manuel Talón Cubillo. IP IRNAS: José M. Colmenero-Flores.
Alteraciones biogeoquímicas mediadas por aves acuáticas en ecosistemas terrestres mediterráneos” (BIOGEOBIRD) (P09-RNM-4987). 2011-2013. Junta de Andalucía, Proyectos de Excelencia. IP: Luis V. García Fernández. Participante: José M. Colmenero-Flores.
Tolerancia al déficit hídrico en plantas basada en la regulación de la homeostasis del anión Cl- y el nivel de ploidía, CLOROPLOID (AGL2009-08339). 2010-2013. Plan Nacional I+D+I 2009. IP: José M. Colmenero-Flores.
Estudio funcional de algunas hidrofilinas vegetales y el impacto de su expresión heteróloga en la tolerancia a sequía (CONACYT-132258). 2010-2013. Consejo Nacional de Ciencia y Tecnología (México). IP: Alejandra Covarrubias Robles. Participante: Miguel A. Rosales Villegas.

Caracterización funcional de factores transcripcionales involucrados en la respuesta a déficit hídrico en frijol: análisis de su potencial como herramientas para conferir o seleccionar resistencia a sequia (CONACYT-83553). 2009-2010. Consejo Nacional de Ciencia y Tecnología (México). IP: Francisco Campos. Participante: Miguel A. Rosales Villegas.
Un nuevo mecanismo de tolerancia a déficit hídrico en plantas superiores: caracterización fisiológica y molecular (PIE-200840I222). 2008-2009. CSIC-I3. IP: José M. Colmenero-Flores.
Análisis genómico y fenotípico de la variabilidad genética de los cítricos en relación con la tolerancia frente a los estreses abióticos. 2007-2008. PNI+D+I, Ministerio de Educación y Ciencia. IP: Manuel Talón Cubillo. Participante: José M. Colmenero-Flores.
Citrus rootstock breeding for efficient water and nutrient use (FP6-2003-INCO-MPC-015453). 2006-2008. European Commission. IP: Patrick Ollitrault. Participante: José M. Colmenero-Flores.
Tolerancia a la salinidad y al déficit hídrico en cítricos: bases fisiológicas y moleculares y criterios agronómico (AGL2003-08502-C04-01). 2004-2006. Plan Nacional de I+D+I. Ministerio de Ciencia y Tecnología. IP: Manuel Talón Cubillo. Participante: José M. Colmenero-Flores.
Bac-ends sequencing for the physical mapping of the Citrus clementina nuclear genome. GENOSCOPE (Gobierno francés). IPs: Manuel Talón Cubillo (España) / Raphael Morillon (Francia). Participante: José M. Colmenero-Flores.
Análisis nutricional de seis especies de quercíneas de interés forestal en Andalucía y propuestas para su gestión. 2004-2007. Proyecto de Excelencia, Junta de Andalucía. IP: Luis Romero Monreal. Participante: Miguel A. Rosales Villegas.
Evaluación ambiental y mejoras de la calidad de la producción y eficiencia de uso de recursos del agrosistema invernaderos mediterráneos (I+D+I INIA RTA03-096-C5-1). 2003-2006. IP: Nicolás Castilla. Participante: Miguel A. Rosales.
Mejora de la tolerancia salina de cítricos potenciando la compartimentalización de NaCl en portainjertos” (BIO-2000-0174-P4-02). 2002-2004. Ministerio de Ciencia y Tecnología, Plan Nacional de I+D+I, CICyT-P4. IP (Viveros Sevilla S.A.): José M. Colmenero-Flores.
Obtención de portainjertos de cítricos resistentes a salinidad mediante transformación con un gen excluidor de sodio (FIT-010000-2001-134), 2001. Ministerio de Ciencia y Tecnología, Plan Nacional de I+D+I, PROFIT. IP: José M. Colmenero-Flores.
Producción de cítricos, olivos y frutales de hueso mediante cultivo in-vitro de tejidos vegetales. Desarrollo de técnicas moleculares de certificación varietal y control de la estabilidad genética” (FIT-010000-2001-78). 2001-2002. Ministerio de Ciencia y Tecnología, Plan Nacional de I+D+I, PROFIT. IP: José M. Colmenero-Flores.

Estudio de genes y proteínas involucradas en la respuesta a déficit hídrico en plantas superiores y levadura (26242N). 1998-2000. Consejo Nacional de Ciencia y Tecnología, México. IP: Alejandra Covarrubias Robles. Participante: José M. Colmenero-Flores.
El papel de las proteínas LEA en la tolerancia a la sequía en plantas. 1997-1999. Consejo Nacional de Ciencia y Tecnología (México) y KFA (Alemania). IPs: Alejandra Covarrubias Robles y Dorothea Bartles. Participante: José M. Colmenero-Flores.
Caracterización de genes involucrados por déficit de agua en Phaseolus vulgaris (0054N9106). 1994-1996. Consejo Nacional de Ciencia y Tecnología, México. IP: Alejandra Covarrubias Robles. Participante: José M. Colmenero-Flores.
Aislamiento y caracterización de genes inducidos en la respuesta a déficit hídrico en Phaseolus vulgaris (0131PN). 1993-1995. Consejo Nacional de Ciencia y Tecnología, México. IP: Alejandra Covarrubias Robles. Participante: José M. Colmenero-Flores.

Publications

Lucas M, Diaz-Espejo A, Romero-Jimenez D, Peinado-Torrubia P, Delgado-Vaquero A, Álvarez R, Colmenero-Flores JM & Rosales MA Chloride reduces plant nitrate requirement and alleviates low nitrogen stress symptoms Plant Physiology and Biochemistry, Submitted
Pons-Perpinyà J, Peinado-Torrubia P, Colmenero-Flores, JM & Gago J. The role of the “forgotten” elements Magnesium and Chloride for improving crop WUE Theoretical and Experimental Plant Physiology, Submitted
Garcia-Rodriguez AF, Moreno-Racero FJ, Knicker H, Álvarez R, Colmenero-Flores JM, Rosales MA. Biochar enhances nitrogen use efficiency by promoting nitrate assimilation in lettuce plants. Submitted
Peinado-Torrubia P, Álvarez R, Lucas M, Franco-Navarro JD, Durán-Gutiérrez FJ, Colmenero-Flores JM & Rosales MA (2023) Nitrogen assimilation and photorespiration become more efficient under chloride nutrition as a beneficial macronutrient Frontiers in Plant Science, 13:1058774.
Cakmak I, Brown PH, Colmenero-Flores JM, Husted S, Zhao FJ (2023). Micronutrients In: Marschner’s Mineral Nutrition of Plants 4th Ed, Chapter 7. Rengel Z, Cakmak L and White P (Ed). Academic Press. ISBN 9780128121634
Díaz-Rueda P, Peinado-Torrubia P, Durán-Gutiérrez FJ, Alcántara-Romano P, Aguado A, Capote N, Colmenero-Flores, JM (2022). Avoidant/resistant rather than tolerant olive rootstocks are more effective in controlling Verticillium wilt. Frontiers in Plant Science 13:1032489.
Díaz-Rueda P, Cantos-Barragán M, Colmenero-Flores JM (2021) Growth quality and development of olive plants cultured in vitro under different illumination regimes. Plants 10:2214.
Díaz-Rueda P, Aguado A, Romero-Cuadrado L, Capote N, Colmenero-Flores JM (2021) Wild olive genotipes as a valuable source of resistance to defoliating Verticillium dahliae. Frontiers in Plant Science 12:662060.
Franco-Navarro JD, Díaz-Rueda P, Rivero-Núñez CM, Brumós J, Rubio-Casal AE, de Cires A, Colmenero-Flores JM, Rosales MA (2021) Chloride nutrition improves drought resistance by enhancing water deficit avoidance and tolerance mechanisms. Journal of Experimental Botany 72:5246-5261.

Díaz-Rueda P, Franco-Navarro JD, Messora R, Espartero J, Rivero-Núñez CM, Aleza P, Capote N, Cantos M, García-Fernández JL, de Cires A, Belaj A, León L, Besnard G, Colmenero-Flores JM (2020) SILVOLIVE, a germplasm collection of wild subspecies with high genetic variability as a source of rootstocks and resistance genes for olive breeding. Frontiers in Plant Science 11:629.
Rosales MA* & Franco-Navarro JD*, Peinado-Torrubia P, Díaz-Rueda P, Alvarez R, Colmenero-Flores JM (2020) Chloride improves nitrate utilization and NUE in plants. Frontiers in Plant Science 11:442.
Gallego-Tévar B, Peinado-Torrubia P, Álvarez R, Gandullo J, Grewell BJ, Figueroa E, Castillo JM (2020) Changes to the functional traits of phosphoenolpyruvate carboxylase following hybridization in C-4 halophytes. Physiologia Plantarum 169:83–98.
León L, Díaz-Rueda P, Belaj A, De la Rosa R, Carrascosa C, Colmenero-Flores JM (2020) Evaluation of early vigor traits in wild olive germplasm. Scientia Horticulturae 264:109157.
Nieves-Cordones M, García-Sánchez F, Pérez-Pérez J, Colmenero-Flores JM, Rubio F, Rosales MA (2019) Coping with water shortage: An update on the role of K⁺, Cl^–, and water membrane transport mechanisms on drought resistance. Frontiers in Plant Science 10:1619.
Colmenero-Flores JM, Franco-Navarro JD, Cubero-Font P, Peinado-Torrubia P, Rosales MA (2019) Chloride as a beneficial macronutrient in higher plants: New roles and regulation. International Journal of Molecular Sciences 20:4686.
Hernandez-Santana V, Diaz-Rueda P, Diaz-Espejo A, Raya-Sereno MD, Gutiérrez-Gordillo S, Montero A, Perez-Martin A, Colmenero-Flores JM, Rodriguez-Dominguez CM (2019) Hydraulic traits emerge as relevant determinants of growth patterns in wild olive genotypes under water stress. Frontiers in Plant Science 10:291.
Franco-Navarro JD^* & Rosales MA^*, Cubero-Font P, Calvo P, Álvarez R, Díaz-Espejo A, Colmenero-Flores JM (2019) Chloride as macronutrient increases water use efficiency by anatomically-driven reduced stomatal conductance and increased mesophyll diffusion to CO₂. The Plant Journal 99:815-831. ^*Co-first authors.
Rosales MA, Maurel C, Nacry P (2019) Abscisic acid coordinates dose-dependent developmental and hydraulic responses of roots to water deficit. Plant Physiology 180:2198-2211.
Cubero-Font P*, Maierhofer T*, Jaslan J, Rosales MA, Espartero J, Díaz-Rueda P, Müller, HM, Hürter A-L, AL-Rasheid KAS, Marten I, Hedrich R, Colmenero-Flores JM, Geiger D (2016) Silent S-Type Anion Channel Subunit SLAH1 Gates SLAH3 Open for Chloride Root-to-Shoot Translocation. Current Biology 26:2213-2220.
Franco-Navarro JD, Brumós J, Rosales MA, Cubero-Font P, Talón, M, Colmenero-Flores JM (2016) Chloride regulates leaf cell size and water relations in tobacco plants. Journal of Experimental Botany 67:873-891.
Paneque M, De la Rosa JM, Franco-Navarro JD, Colmenero-Flores JM, Knicker H (2016) Effect of biochar amendment on morphology, productivity and water relations of sunflower plants under non-irrigation conditions. Catena 147:280-287.

Pérez-Pérez JG, Gómez-Gómez AG, Botía P, Brumós J, Talón M, Colmenero-Flores JM (2015) Transcriptional profile analysis of young and mature leaves of citrus trees acclimated to salinity. Acta Horticulturae 1065:1359-1370.
Terol J, Carbonell J, Alonso R, Tadeo FR, Herrero-Ortega A, Ibañez V, Muñoz JV, López-García A, Estornell LH, Colmenero-Flores JM, et al. (2015) Sequencing of 150 citrus varieties: Linking genotypes to phenotypes. Acta Horticulturae 1065:585-590.
Colmenero-Flores JM & Rosales MA (2014) Interaction between salt and heat stress: when two wrongs make a right. Plant Cell Environment 37:1042–1045.
Aguado A, Capote N, Romero F, Dodd IC, Colmenero-Flores JM (2014) Physiological and gene expression responses of sunflower (Helianthus annuus L.) plants differ according to irrigation placement. Plant Science 227:37-44.
Rodríguez-Valentín R, Campos F, Battaglia M, Solórzano RM, Rosales MA, Covarrubias AA. (2014) Group 6 LEA proteins in monocotyledoneous plants: genomic organization and transcript accumulation patterns in response to stress in Oryza sativa. Plant Molecular Biology Reporter 32:198–208.
Allario T, Brumos J, Colmenero-Flores JM, Iglesias DJ, Pina JA, Navarro L, et al. (2013) Tetraploid Rangpur lime rootstock increases drought tolerance via enhanced constitutive root abscisic acid production. Plant Cell and Environment 36:856-868.
Rosales MA, Cuellar-Ortiz SM, Arrieta-Montiel MP, Acosta-Gallegos J, Covarrubias AA (2013) Physiological traits related to terminal drought resistance in common bean (Phaseolus vulgaris). Journal of the Science of Food & Agriculture 93:324-331.
Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Rosales MA, Blasco B, Rios JJ, Romero L, Blumwald E, Ruiz JM (2012) Ammonium formation and assimilation in PSARK::IPT tobacco transgenic plants under low N. Journal of Plant Physiology 169:157-162.
Rosales MA, Ocampo E, Rodríguez-Valentín R, Olvera-Carrillo Y, Acosta-Gallegos J, Covarrubias AA (2012) Physiological analysis of common bean (Phaseolus vulgaris) cultivars uncovers characteristics related to terminal drought resistance. Plant Physiology and Biochemistry 56:24-34.
Allario T, Brumos J, Colmenero-Flores JM, Tadeo F, Froelicher Y, Talon M, et al. (2011) Large changes in anatomy and physiology between diploid Rangpur lime (Citrus limonia) and its autotetraploid are not associated with large changes in leaf gene expression. Journal of Experimental Botany 62:2507-2519.
Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Rosales MA, Blasco B, Rios JJ, Romero L, Blumwald E, Ruiz JM (2011) Cytokinin-dependent Improvement in Transgenic PSARK::IPT Tobacco under N Deficiency. Journal of Agriculture and Food Chemistry 59:10491-10495.
Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Rosales MA, Blasco B, Rios JJ, Romero L, Blumwald E, Ruiz JM (2011) Effect of cytokinins on oxidative stress in tobacco plants under nitrogen deficiency. Environmental and Experimental Botany 72:167-173.
Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Ríos JJ, Blasco B, Rosales MA, Melgarejo R, Romero L, Ruiz JM (2011) Ammonia production and assimilation: its importance as mechanism tolerance during water deficit in tomato plants. Journal of Plant Physiology 168:816-823.
Talon M, Cercos M, Iglesias DJ, Colmenero-Flores JM, Ibáñez V, Brumos J, et al. (2011) Citrus genomics and breeding: Identification of candidate genes by the use of mutants and microarrays. Acta Horticulturae 892:19-26.
Rosales MA, Cervilla LM, Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Blasco B, Ríos JJ, Soriano T, Castilla N, Romero L, Ruiz JM (2011) The effect of environmental conditions on nutritional quality of cherry tomato fruits: evaluation of two experimental Mediterranean greenhouses. Journal of the Science of Food & Agriculture 91, 152-162.
Blasco B, Ríos JJ, Leyva R, Cervilla LM, Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Rosales MA, Ruiz JM, Romero L (2011) Does iodine biofortification affect oxidative metabolism in lettuce plants? Biological Trace Element Research 142:831-842.

Brumós J, Talón M, Bouhlal R, Colmenero-Flores JM (2010) Cl- homeostasis in includer and excluder citrus rootstocks: Transport mechanisms and identification of candidate genes. Plant Cell and Environment 33:2012-2027.
Ríos JJ, Blasco B, Cervilla LM, Rubio-Wilhelmi MM, Rosales MA, Sánchez-Rodríguez E, Romero L, Ruiz JM (2010) Nitrogen-use efficiency in relation to different forms and application rates of Se in lettuce plants. Journal of Plant Growth Regulation 29:164-170.
Ríos JJ, Blasco B, Rosales MA, Sánchez-Rodríguez, Leyva R, Cervilla LM, Romero L, Ruiz JM (2010) Response of nitrogen metabolism in lettuce plants subjected to different doses and forms of selenium. Journal of the Science of Food & Agriculture 90:1914-1919.
Blasco B, Ríos JJ, Cervilla LM, Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Rosales MA, Ruiz JM, Romero L (2010) Photorespiration process and nitrogen metabolism in lettuce plants (Lactuca sativa): induced changes in response to iodine biofortification. Journal of Plant Growth Regulation 29:477-486.
Gimeno J, Gadea J, Forment J, Pérez-Valle J, Santiago J, Martínez-Godoy MA, Colmenero-Flores JM, et al. (2009) Shared and novel molecular responses of mandarin to drought. Plant Molecular Biology 70:403-420.
Brumós J, Colmenero-Flores JM, Conesa A, Izquierdo P, Sánchez G, Iglesias DJ, et al. (2009) Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks. Functional Integrative Genomics 9:293-309.
Sánchez-Rodríguez E, Rubio-Wilhelmi MM, Cervilla LM, Blasco B, Ríos JJ, Rosales MA, Romero L, Ruiz JM (2009) Genotypic differences in some physiological parameters symptomatic for oxidative stress under drought in tomato plants. Plant Science 178:30-40.
Cervilla LM, Blasco B, Ríos JJ, Rosales MA, Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Romero L, Ruiz JM (2009) Response of nitrogen metabolism to boron toxicity in tomato plants. Plant Biology 11:671-677.
Rosales MA, Cervilla LM, Ríos JJ, Blasco B, Sánchez-Rodríguez E, Romero L, Ruiz JM (2009) Environmental conditions affect pectin solubilization in cherry tomato fruits grown in two experimental Mediterranean greenhouses. Environmental & Experimental Botany 67:320-327.
Cervilla LM, Rosales MA, Rubio-Wilhelmi MM, Blasco B, Ríos JJ, Romero L, Ruiz JM (2009) Involvement of lignification and membrane permeability in the tomato root response to boron toxicity. Plant Science 176:545-552.
Rosales MA, Ríos JJ, Cervilla LM, Rubio-Wilhelmi MM, Blasco B, Ruiz JM, Romero L (2009) Environmental conditions in relation to stress in cherry tomato fruits in two experimental Mediterranean greenhouses. Journal of the Science of Food & Agriculture 89, 735-742.
Ríos JJ, Blasco B, Cervilla LM, Rosales MA, Sánchez-Rodríguez E, Romero L, Ruiz JM (2009) Production and detoxification of H₂O₂ in lettuce plants exposed to selenium. Annals of Applied Biology 154:107-116.
Tadeo FR, Cercós M, Colmenero-Flores JM, Iglesias DJ, Naranjo MA, Ríos G, et al. (2008) Molecular Physiology of Development and Quality of Citrus. Advances in Botanical Research 47:147-223.
López-Carrión AI, Castellano R, Rosales MA, Ruiz JM, Romero L (2008) Role of nitric oxide under saline stress: implications on proline metabolism. Biologia Plantarum 52:587-591.
Ríos JJ, Rosales MA, Blasco B, Cervilla LM, Romero L, Ruiz JM (2008) Biofortification of Se and induction of the antioxidant capacity in lettuce plants. Scientia Horticulturae 116:248-255.
Iglesias DJ, Cercós M, Colmenero-Flores JM, Naranjo MA, Ríos G, Carrera E, et al. (2007) Physiology of citrus fruiting. Brazilian Journal of Plant Physiology 19:333-362.
Rosales MA, Rubio-Wilhelmi MM, Castellano R, Castilla N, Ruiz JM, Romero L (2007) Sucrolytic activities in cherry tomato fruits in relation to temperature and solar radiation. Scientia Horticulturae 113:244-249.
Colmenero-Flores JM, Martínez G, Gamba G, Vázquez N, Iglesias DJ, Brumós J, et al. (2007) Identification and functional characterization of cation-chloride cotransporters in plants. Plant Journal 50:278-292.
Rosales MA, Rios JJ, Castellano R, López-Carrión AI, Romero L, Ruiz JM (2007) Proline metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of Horticultural Science & Biotechology 82:739-744.
Rosales MA, Ruiz JM, Hernández J, Soriano T, Castilla N, Romero L (2006) Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of the Science of Food & Agriculture 86:1545-1551.
Ruiz JM, Ríos JJ, Rosales MA, Rivero RM, Romero L (2006) Grafting between tobacco plants to enhance salinity tolerance. Journal of Plant Physiology 163:1229-1237.

Reyes JL, Rodrigo M, Colmenero-Flores JM, Gil J, Garay-Arroyo A, Campos F, et al. (2005) Hydrophilins from distant organisms can protect enzymatic activities from water limitation effects in vitro. Plant Cell and Environment 28:709-718.
Forment J, Gadea J, Huerta L, Abizanda L, Agusti J, Alamar S, Colmenero-Flores JM, et al. (2005) Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies. Plant Molecular Biology 57):375-391.
Campos-Álvarez F, Cruz-García F, Torres-Espinosa A, Sánchez-Jiménez M, Colmenero-Flores JM, Smith-Espinoza C, et al. (2002) Expression of late embryogenesis abundant (lea) protein codifying genes during osmopriming of maize and bean seeds. Agrociencia 36:461-470.
Garay-Arroyo A, Colmenero-Flores JM, Garciarrubio A, Covarrubias AA. (2000) Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. Journal of Biological Chemistry 275:5668-5674.
Colmenero-Flores JM, Moreno LP, Smith CE, Covarrubias AA. (1999) Pvlea-18, a member of a new late-embryogenesis-abundant protein family that accumulates during water stress and in the growing regions of well-irrigated bean seedlings. Plant Physiology 120:93-103.
Villanueva MA, Campos F, Díaz C, Colmenero-Flores JM, Dantán E, Sánchez F, et al. (1999) Actin expression in germinating seeds of Phaseolus vulgaris L. Planta 207:582-589.
Porta H, Rueda-Benítez P, Campos F, Colmenero-Flores JM, Colorado JM, Carmona MJ, et al. (1999) Analysis of lipoxygenase mRNA accumulation in the common bean (Phaseolus vulgaris L.) during development and under stress conditions. Plant Cell Physiology 40:850-858.
Folch-Mallol JL, Manyani H, Marroquí S, Sousa C, Vargas C, Nava N, Colmenero-Flores JM, et al. (1998) Sulfation of nod factors via nodHPQ is nodD independent in Rhizobium tropici CIAT899. Molecular Plant-Microbe Interactions 11:979-987.
Colmenero-Flores JM, Campos F, Garciarrubio A, Covarrubias AA. (1997) Characterization of Phaseolus vulgaris cDNA clones responsive to water deficit: Identification of a novel late embryogenesis abundant-like protein. Plant Molecular Biology 35:393-405.